Lozinov A B, Glazunova L M, Ermakova I T
Mikrobiologiia. 1976 Jan-Feb;45(1):33-40.
The activity of key enzymes of the citrate (CC), glyoxylate (GC), and pentose phosphate cycles (PPC) was determined in the cells of Candida lipolytica and Candida tropicalis growing on hexadecane and glucose. The activity of isocitrate lyase and malate synthase (enzymes of GC) was very high in hexadecane grown cells but was almost absent in "glucose" grown. The activity of citrate synthase and aconitase was 2-4 times and 1.5-2 times, respectively, higher in the cells, grown on n-alkane. The activity of citrate synthase of "hexadecane" and "glucose" yeasts was higher than the activity of other enzymes of CC (aconitase and isocitrate dehydrogenase), especially in the "hexadecane" cells. The activity of the key enzymes of PPC was almost the same in the yeast cells, grown on "hexadecane" and "glucose". Possible factors causing differences in the activities of the enzymes of GC and CC are discussed. Yeast organisms incapable to grow on n-alkanes were found to be able to grow on acetate (43 strains belonging to 35 yeast species have been studied) and to have a high activity of isocitrate lyase. Therefore, the absence of growth of many yeast strains on n-alkanes is not caused by the absence of the activity of enzymes of GC in these strains.
测定了在十六烷和葡萄糖上生长的解脂假丝酵母和热带假丝酵母细胞中柠檬酸循环（CC）、乙醛酸循环（GC）和磷酸戊糖途径（PPC）关键酶的活性。在以十六烷为碳源生长的细胞中，异柠檬酸裂解酶和苹果酸合酶（GC途径的酶）的活性非常高，但在以“葡萄糖”为碳源生长的细胞中几乎没有活性。柠檬酸合酶和乌头酸酶的活性在以正构烷烃为碳源生长的细胞中分别高出2 - 4倍和1.5 - 2倍。“十六烷”和“葡萄糖”酵母的柠檬酸合酶活性高于CC途径的其他酶（乌头酸酶和异柠檬酸脱氢酶）的活性，尤其是在“十六烷”细胞中。PPC途径关键酶的活性在以“十六烷”和“葡萄糖”为碳源生长的酵母细胞中几乎相同。讨论了导致GC和CC途径酶活性差异的可能因素。发现不能在正构烷烃上生长的酵母菌株能够在乙酸盐上生长（研究了属于35个酵母物种的43个菌株），并且具有较高的异柠檬酸裂解酶活性。因此，许多酵母菌株不能在正构烷烃上生长不是因为这些菌株中缺乏GC途径酶活性。
