Morana Alessandra, Stiuso Paola, Colonna Giovanni, Lamberti Monica, Cartenì Maria, De Rosa Mario
Istituto di Biochimica delle Proteine, CNR, via P. Castellino 111, 80131 Naples, Italy.
Biochim Biophys Acta. 2002 Nov 14;1573(2):105-8. doi: 10.1016/s0304-4165(02)00333-1.
S-adenosyl-L-methionine (SAM), an important metabolic intermediate of mammals, is a well-known therapeutic agent. The molecule is chemically unstable, both in solution and in dry state, and forms different degradation products. Because the chemical instability represents a real problem during the preparation of therapeutic formulations, we investigated the capacity of some sugars to improve the SAM stability over time. In the present work, we demonstrated that the disaccharide trehalose exercises a protective effect towards the lyophilized SAM slackening its degradation (65% of SAM was detected after 50 days at 37 degrees C). A parallel study, performed to stabilize the SAM into lyophilized yeast cells enriched in the sulfonium compound, assessed the positive effect of trehalose also in whole cells, but in lesser measure.
S-腺苷-L-甲硫氨酸（SAM）是哺乳动物重要的代谢中间体，是一种广为人知的治疗剂。该分子在溶液和干燥状态下化学性质均不稳定，会形成不同的降解产物。由于化学不稳定性在治疗制剂制备过程中是一个实际问题，我们研究了一些糖类随时间提高SAM稳定性的能力。在本研究中，我们证明二糖海藻糖对冻干的SAM具有保护作用，减缓其降解（在37℃下50天后检测到65%的SAM）。一项平行研究旨在将SAM稳定在富含锍化合物的冻干酵母细胞中，评估了海藻糖在全细胞中的积极作用，但程度较小。
