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真空泡沫干燥：一种用于生物分子保存的冻干替代方法。

Vacuum foam drying: an alternative to lyophilization for biomolecule preservation.

作者信息

Jangle R D, Pisal S S

机构信息

Department of Pharmaceutical Biotechnology, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune-411 038, India.

出版信息

Indian J Pharm Sci. 2012 Mar;74(2):91-100. doi: 10.4103/0250-474X.103837.

DOI:10.4103/0250-474X.103837
PMID:23325988
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3546342/
Abstract

Vacuum foam drying is evaluated as an alternative for lyophilization for enhanced process and storage stability of bovine serum albumin. The protein protective efficiency of different stabilizers was compared in vacuum foam drying and lyophilization. Sucrose mixtures produced better foam characters than mannitol. Unlike calcium lactate, incorporation of polyvinyl pyrrolidone to sucrose synergistically enhanced the recovery of bovine serum albumin. The conformational stability and bovine serum albumin content further increased with sodium phosphate as compared to potassium phosphate. All sucrose mixtures, except calcium lactate showed large α-helix amide-I band at approximately 1656 cm(-1). The amorphous powder diffraction in case of sodium phosphate monobasic mixture retained maximum bovine serum albumin content. The crystallization of similar mixtures in lyophilization reduced its bovine serum albumin content. Vacuum foam drying showed better processing and storage stability of bovine serum albumin than lyophilization process. Hence vacuum foam drying is short, simple and industrially economical process for biomolecules preservation.

摘要

真空泡沫干燥作为冻干的替代方法进行了评估，以提高牛血清白蛋白的加工和储存稳定性。比较了不同稳定剂在真空泡沫干燥和冻干过程中的蛋白质保护效率。蔗糖混合物产生的泡沫特性比甘露醇更好。与乳酸钙不同，将聚乙烯吡咯烷酮加入蔗糖中可协同提高牛血清白蛋白的回收率。与磷酸钾相比，磷酸钠可进一步提高牛血清白蛋白的构象稳定性和含量。除乳酸钙外，所有蔗糖混合物在约1656 cm(-1)处均显示出较大的α-螺旋酰胺-I带。磷酸二氢钠混合物的无定形粉末衍射保留了最高的牛血清白蛋白含量。冻干过程中类似混合物的结晶降低了其牛血清白蛋白含量。真空泡沫干燥显示出比冻干过程更好的牛血清白蛋白加工和储存稳定性。因此，真空泡沫干燥是一种用于生物分子保存的简短、简单且工业上经济的过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12a2/3546342/ae78429f6826/IJPhS-74-91-g012.jpg
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Moisture-induced aggregation of lyophilized proteins in the solid state.固态下冻干蛋白质的水致聚集。
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Vacuum foam drying for preservation of LaSota virus: effect of additives.用于保存拉索塔病毒的真空泡沫干燥法：添加剂的作用
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The Application of Light-Assisted Drying to the Thermal Stabilization of Nucleic Acid Nanoparticles.光辅助干燥在核酸纳米颗粒热稳定化中的应用。
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