Hawkins R A, Freedman B, Marshall A, Killen E
Br J Cancer. 1975 Aug;32(2):179-85. doi: 10.1038/bjc.1975.147.
Radioimmunoassay methods for the determination of oestradiol-17 beta and prolactin have been examined for their reliability and applied to the measurement of hormone concentrations in the plasmas from male and female rats. Prolactin was detectable in all samples (greater than 7 ng RP-1 ng/ml) but the concentration of oestradiol-17 beta was below the sensitivity of the method (greater than 0-10 ng/100 ml) in ovariectomized females. Plasma oestradiol-17 beta concentration rose gradually from metoestrus to proestrus and fell to barely detectable levels in oestrus. Plasma prolactin concentration was very variable even for rats in the same stage of the oestrous cycle but values were minimal in the afternoon of diestrus and a surge in secretion occurred in the afternoon of proestrus. In addition to the stage of the oestrous cycle, the prolactin concentration was influenced by mode of blood collection, degree of haemolysis and choice of serum or plasma. There was no correlation between the concentration of prolactin and that of oestradiol-17 beta in the same sample of plasma.
已对用于测定雌二醇 - 17β和催乳素的放射免疫分析方法的可靠性进行了检验，并将其应用于测量雄性和雌性大鼠血浆中的激素浓度。在所有样本中均能检测到催乳素（大于7 ng RP - 1 ng/ml），但在去卵巢雌性大鼠中，雌二醇 - 17β的浓度低于该方法的灵敏度（大于0 - 10 ng/100 ml）。血浆雌二醇 - 17β浓度从间情期到动情前期逐渐升高，在发情期降至几乎检测不到的水平。即使对于处于发情周期同一阶段的大鼠，血浆催乳素浓度也变化很大，但在动情后期下午的值最小，而在动情前期下午出现分泌高峰。除发情周期阶段外，催乳素浓度还受采血方式、溶血程度以及血清或血浆的选择影响。在同一血浆样本中，催乳素浓度与雌二醇 - 17β浓度之间没有相关性。
