Shaikh A A, Shaikh S A
Endocrinology. 1975 Jan;96(1):37-44. doi: 10.1210/endo-96-1-37.
This study was undertaken to determine the patterns of estrogen and progestin secretion by the adrenal and the ovary and to relate these to the levels of steroids and gonadotropins found in peripheral plasma. A total of 70 rats were used in this study. Adrenal venous (ADVB) and ovarian venous blood (OVB) were collected by concurrent cannulation and peripheral blood (PB) was obtained from the abdominal aorta. Estrone (E1), estradiol (E2), PROGESTERONE (P) and 17alpha-hydroxyprogesterone (17alpha-OHP) were measured by radioimmunoassay. The concentration of E2 in general was higher in ADVB than in OVB. Although increased levels of E2 in ADVB were found on the afternoon of proestrus (1527-2634 pg/ml), they were not significantly different from those recorded at other times. In contrast E2 in OVB was lowest at 1000-1200 hr of estrus (225 pg/ml) and highest at 1000-2000 hr of proestrus (1190-1605 pg/ml). The E2 in PB followed the pattern seen in OVB; the values ranged from 80-211 pg/ml. P concentration in general was higher in ADVB than in OVB. The lowest concentration of P in ADVB was between 0200-0400 hr of estrus (183 ng/ml) and the highest between 1400-1600 hr of proestrus (580 ng/ml) and 1000-1200 hr of estrus (591 ng/ml). The lowest concentration of P in the OVB was between 1600-1800 hr of diestrus (42 ng/ml) and the highest between 0200-0400 hr of estrus (466 ng/ml). In the PB, P reflected changes in adrenal as well as ovarian secretion; the values ranged fron 7-25 ng/ml. E1 in ADVB was in general lower than in OVB, and there were no significant changes at any stage of the cycle. In the OVB, however, E1 showed a pattern similar to that of E2. Levels of E1 in PB were very low and failed to show any significant changes. 17alpha-OHP was high in ADVB, although there were no significant changes during the cycle; PB levels also did not show any significant changes. In the OVB a peak of 17alpha-OHP was recorded between 0200-0400 hr on the day of estrus. The LH and FSH levels showed increases after 1600 hr on the day of proestrus and this was in agreement with earlier studies. Since the adrenal secretes all of the 4 steroids studied, it is reasonable to assume that it does exert some effect in the pituitary-gonadal axis. A role of adrenal P for the facilitation of the preovulatory LH release has been indicated and the increased secretion of adrenal P between 1400-1600 hr of proestrus supports this possibility.
本研究旨在确定肾上腺和卵巢分泌雌激素和孕激素的模式，并将其与外周血浆中类固醇和促性腺激素的水平联系起来。本研究共使用了70只大鼠。通过同时插管收集肾上腺静脉血（ADVB）和卵巢静脉血（OVB），并从腹主动脉获取外周血（PB）。采用放射免疫分析法测定雌酮（E1）、雌二醇（E2）、孕酮（P）和17α-羟孕酮（17α-OHP）。一般来说，ADVB中E2的浓度高于OVB。虽然在发情前期下午ADVB中E2水平升高（1527 - 2634 pg/ml），但与其他时间记录的水平无显著差异。相比之下，OVB中E2在发情期1000 - 1200时最低（225 pg/ml），在发情前期1000 - 2000时最高（1190 - 1605 pg/ml）。PB中的E2呈现出与OVB中相似的模式；其值范围为80 - 211 pg/ml。一般来说，ADVB中P的浓度高于OVB。ADVB中P的最低浓度在发情期0200 - 0400时（183 ng/ml），最高浓度在发情前期1400 - 1600时（580 ng/ml）和发情期1000 - 1200时（591 ng/ml）。OVB中P的最低浓度在间情期1600 - 1800时（42 ng/ml），最高浓度在发情期0200 - 0400时（466 ng/ml）。在PB中，P反映了肾上腺以及卵巢分泌的变化；其值范围为7 - 25 ng/ml。ADVB中E1一般低于OVB，且在周期的任何阶段均无显著变化。然而，在OVB中，E1呈现出与E2相似的模式。PB中E1的水平非常低，未显示出任何显著变化。ADVB中17α-OHP较高，尽管在周期中无显著变化；PB水平也未显示出任何显著变化。在OVB中，发情当天0200 - 0400时记录到17α-OHP的峰值。促黄体生成素（LH）和促卵泡生成素（FSH）水平在发情前期当天1600时后升高，这与早期研究一致。由于肾上腺分泌所研究的所有4种类固醇，因此有理由假设它在垂体-性腺轴中确实发挥了某种作用。肾上腺P对排卵前LH释放的促进作用已得到提示，发情前期1400 - 1600时肾上腺P分泌增加支持了这种可能性。
