Zhang Liyao, Huang Weihua, Wang Zongli, Cheng Jieke
Department of Chemistry, Wuhan University, China.
Anal Sci. 2002 Oct;18(10):1117-20. doi: 10.2116/analsci.18.1117.
Capillary zone electrophoresis was employed for the determination of histamine using end-column amperometric detection with a carbon fiber microelectrode, at a constant potential. The optimum conditions of separation and detection were 10 mmol/L phosphate buffer, pH 5.6 for the buffer solution, 15 kV for the separation voltage, and 1.35 V (versus SCE) for the detection potential. The linear range was from 6.3 x 10(-7) to 1.5 x 1(-5) mol/L with the regression coefficient of 0.9997, and the detection limit was 4.0 x 10(-7) mol/L (S/N = 3). The proposed method was successfully applied to the direct determination of histamine in the beer samples without any sample clean-up procedures.
采用毛细管区带电泳法，使用碳纤维微电极在恒电位下进行柱端安培检测来测定组胺。分离和检测的最佳条件为：缓冲溶液为10 mmol/L磷酸盐缓冲液，pH 5.6；分离电压为15 kV；检测电位为1.35 V（相对于饱和甘汞电极）。线性范围为6.3×10⁻⁷至1.5×10⁻⁵ mol/L，回归系数为0.9997，检测限为4.0×10⁻⁷ mol/L（信噪比 = 3）。该方法成功应用于啤酒样品中组胺的直接测定，无需任何样品净化程序。
