将普鲁卡因注入腹中内侧下丘脑可抑制在氨基甲酸乙酯麻醉大鼠中由额叶皮质刺激引起的肠脂肪酸结合蛋白激活。

Procaine into the VMH inhibits IBAT activation caused by frontal cortex stimulation in urethane-anesthetized rats.

作者信息

Monda M, Viggiano A, Caserta L, De Luca V

机构信息

Department of Experimental Medicine, Section of Human Physiology, Second University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.

出版信息

Neuroscience. 2002;115(1):79-83. doi: 10.1016/s0306-4522(02)00398-6.

DOI:10.1016/s0306-4522(02)00398-6

PMID:12401323

Abstract

This experiment tested the effect of procaine injection into the ventromedial hypothalamus on the sympathetic and thermogenic activation induced by frontal cortex stimulation. Oxygen consumption, firing rate of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT and colonic temperatures were monitored in fasted male Sprague-Dawley rats before and during 25 min after an electrical stimulation of the frontal cortex. The same variables were monitored in rats with administration of procaine into the ventromedial hypothalamus. The results show that cortical stimulation increases oxygen consumption, sympathetic firing rate, IBAT and colonic temperatures. The increase in sympathetic firing rate was reduced by procaine injection, and the increase in IBAT and colonic temperatures as well as oxygen consumption was fully inhibited by procaine. These findings suggest that the ventromedial nucleus plays an important role in the sympathetic and thermogenic changes induced by cortical stimulation.

摘要

本实验测试了向腹内侧下丘脑注射普鲁卡因对额叶皮质刺激所诱导的交感神经和产热激活的影响。在禁食的雄性斯普拉格-道利大鼠中，于额叶皮质电刺激前及刺激后25分钟内，监测其耗氧量、支配肩胛间棕色脂肪组织（IBAT）的交感神经放电频率，以及IBAT和结肠温度。在向腹内侧下丘脑注射普鲁卡因的大鼠中监测相同的变量。结果显示，皮质刺激可增加耗氧量、交感神经放电频率、IBAT和结肠温度。注射普鲁卡因可降低交感神经放电频率的增加，并且普鲁卡因可完全抑制IBAT和结肠温度以及耗氧量的增加。这些发现表明，腹内侧核在皮质刺激所诱导的交感神经和产热变化中起重要作用。