Monda M, Viggiano An, Viggiano Al, Fuccio F, De Luca V
Department of Experimental Medicine, Section of Human Physiology, Second University of Naples, Naples, Italy.
Physiol Res. 2004;53(5):507-13.
This experiment tested the effect of clozapine on the sympathetic and thermogenic effects induced by orexin A. The firing rates of the sympathetic nerves to interscapular brown adipose tissue (IBAT), along with IBAT and colonic temperatures were monitored in urethane-anesthetized male Sprague-Dawley rats before and for 5 h after an injection of orexin A (1.5 nmol) into the lateral cerebral ventricle. The same procedure was carried out in rats treated with orexin A plus an intraperitoneal administration of clozapine (8 mg/kg bw), an atypical antipsychotic that is largely used in the therapy of schizophrenia. The same variables were monitored in rats with clozapine alone. A group of rats with saline injection served as control. The results show that orexin A increases the sympathetic firing rate, IBAT and colonic temperatures. Clozapine blocks completely the reactions due to orexin A. These findings suggest that clozapine influences strongly the thermogenic role of orexin A. Furthermore, the remarkable hyperthermic role played by orexin A is confirmed.
本实验测试了氯氮平对食欲素A诱导的交感神经和产热效应的影响。在向侧脑室注射食欲素A（1.5纳摩尔）之前及之后5小时，监测了用乌拉坦麻醉的雄性斯普拉格-道利大鼠的肩胛间棕色脂肪组织（IBAT）交感神经放电频率，以及IBAT和结肠温度。在用食欲素A加腹腔注射氯氮平（8毫克/千克体重）处理的大鼠中进行了相同的操作，氯氮平是一种非典型抗精神病药物，大量用于精神分裂症治疗。对单独使用氯氮平的大鼠监测了相同变量。一组注射生理盐水的大鼠作为对照。结果表明，食欲素A可提高交感神经放电频率、IBAT和结肠温度。氯氮平完全阻断了由食欲素A引起的反应。这些发现表明，氯氮平强烈影响食欲素A的产热作用。此外，食欲素A显著的体温升高作用得到了证实。
