Banrezes B, Andrey P, Maschino E, Schirar A, Peytevin J, Rampin O, Maurin Y
Analyse et Modélisation en Imagerie Biologique, I.N.R.A. Jeune Equipe 1060/77, 78352 Jouy-en-Josas, France.
Neuroscience. 2002;115(1):97-109. doi: 10.1016/s0306-4522(02)00405-0.
The purpose of the present investigations was (1) to examine the spatial organization of preganglionic neurons of the sacral parasympathetic nucleus in the lumbosacral spinal cord of male adult rats and (2) to search, in this nucleus, for a possible segregation of sub-populations of neurons innervating the penis or the bladder, respectively. To estimate their spatial organization, neurons of the sacral parasympathetic nucleus were retrogradely labeled by wheat germ agglutinin coupled to horseradish peroxidase applied to the central end of the sectioned pelvic nerve. The sub-populations of lumbosacral neurons innervating the corpus cavernosum of the penis or the dome of the bladder were identified using transsynaptic retrograde labeling by pseudorabies virus injected into these organs in different rats. In both wheat germ agglutinin-labeled and pseudorabies virus-labeled rats, serial coronal sections were cut through the spinal L5-S1 segments. Labeled neurons were revealed by histochemistry (peroxidase experiments) or immunohistochemistry (pseudorabies virus experiments). By means of a three-dimensional reconstruction software developed in our laboratory, three-dimensional models were calculated from each spinal section image series. They revealed the spatial organization of (i) preganglionic neurons and (ii) neurons innervating the bladder or the penis. The different three-dimensional models were subsequently merged into a single one which revealed the segregation, within the sacral parasympathetic nucleus, of the sub-populations of neurons. Neurons labeled by virus injected into the penis extended predominantly from the rostral part of the L6 segment to the rostral part of the S1 segment while those labeled by bladder injections were distributed predominantly from the caudal part of the L6 segment to the caudal part of the S1 segment. These results support the hypothesis of a viscerotopic organization of sacral neurons providing the spinal control of pelvic organs.
（1）检查成年雄性大鼠腰骶脊髓中骶副交感核节前神经元的空间组织；（2）在该核中寻找分别支配阴茎或膀胱的神经元亚群的可能分离。为了估计它们的空间组织，将与辣根过氧化物酶偶联的麦胚凝集素应用于切断的盆神经的中枢端，对骶副交感核的神经元进行逆行标记。通过将伪狂犬病病毒注射到不同大鼠的这些器官中，利用跨突触逆行标记来识别支配阴茎海绵体或膀胱顶部的腰骶神经元亚群。在麦胚凝集素标记的大鼠和伪狂犬病病毒标记的大鼠中，均切取脊髓L5-S1节段的连续冠状切片。通过组织化学（过氧化物酶实验）或免疫组织化学（伪狂犬病病毒实验）显示标记的神经元。借助我们实验室开发的三维重建软件，从每个脊髓切片图像系列计算三维模型。它们揭示了（i）节前神经元和（ii）支配膀胱或阴茎的神经元的空间组织。随后将不同的三维模型合并为一个单一模型，该模型揭示了骶副交感核内神经元亚群的分离。注射到阴茎中的病毒标记的神经元主要从L6节段的头端延伸到S1节段的头端，而膀胱注射标记的神经元主要从L6节段的尾端分布到S1节段的尾端。这些结果支持了提供盆腔器官脊髓控制的骶神经元内脏定位组织的假说。
