Dorofeeva A A, Panteleev S S, Makarov F N
Corticovisceral Physiology Laboratory, St. Petersburg, Russia.
Neurosci Behav Physiol. 2009 Feb;39(2):207-10. doi: 10.1007/s11055-009-9104-z. Epub 2009 Jan 14.
Studies in anesthetized (urethane, 1.5 g/kg, i.p.) cats using retrograde transport of horseradish peroxidase addressed the locations and morphometric characteristics of neurons in the sacral parasympathetic nucleus of the spinal cord innervating the descending colon and rectum. Marker solution was injected beneath the serous membrane of the study areas of the large intestine. Transcardiac perfusion with fixative solution was performed 48 h later and frontal sections of the sacral segments of the spinal cord were prepared; these were processed by the Mesulam method (1978). The results showed that these areas of the large intestine receive innervation from neurons in the sacral parasympathetic nucleus located in spinal cord segments SI, SII, and SIII. The largest number of labeled cells was seen in segment SII. The neurons of this nucleus innervating the study areas of the large intestine formed two longitudinally distributed group (a lateral and a dorsal), the cells of which differed in terms of size and the orientation of the long axis. The largest number of labeled cells was seen in the lateral group.
在使用辣根过氧化物酶逆行运输法对麻醉（腹腔注射1.5 g/kg氨基甲酸乙酯）猫进行的研究中，探讨了支配降结肠和直肠的脊髓骶副交感核中神经元的位置和形态特征。标记溶液被注射到大肠研究区域的浆膜下方。48小时后进行经心灌注固定液，并制备脊髓骶段的额状切片；这些切片采用Mesulam法（1978年）进行处理。结果表明，大肠的这些区域接受来自位于脊髓SⅠ、SⅡ和SⅢ节段的骶副交感核中神经元的支配。在SⅡ节段观察到的标记细胞数量最多。该核中支配大肠研究区域的神经元形成两个纵向分布的组（外侧组和背侧组），两组细胞在大小和长轴方向上存在差异。外侧组中观察到的标记细胞数量最多。
