Farwanah Hany, Neubert Reinhard, Zellmer Sebastian, Raith Klaus
Institute of Pharmaceutics and Biopharmaceutics, Martin Luther University, W-Langenbeck-Str 4, 06120 Halle (S), Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Nov 25;780(2):443-50. doi: 10.1016/s1570-0232(02)00654-2.
The separation of the major stratum corneum lipids, i.e., ceramides, fatty acids, cholesterol and its esters by means of high-performance thin-layer chromatography is hereby presented. The used automated multiple development technique allows the reproducible development of a 17-step solvent gradient also capable of separating seven ceramide classes in the same run. Reliable quantification has been performed after visualisation and densitometric scanning. The present approach is less time and solvent-consuming than previously described procedures. The application to samples obtained by in vivo skin surface extraction with hexane-ethanol (2:1) demonstrates that the method can be routinely used for diagnostic purposes.
本文介绍了通过高效薄层色谱法分离主要角质层脂质，即神经酰胺、脂肪酸、胆固醇及其酯类的方法。所采用的自动多次展开技术能够实现17步溶剂梯度的可重复展开，且在同一运行中还能分离出七种类神经酰胺。在显色和光密度扫描后进行了可靠的定量分析。与先前描述的方法相比，本方法耗时更少且溶剂消耗更低。将该方法应用于通过己烷 - 乙醇（2:1）进行体内皮肤表面萃取获得的样品，结果表明该方法可常规用于诊断目的。
