Schürer N Y, Schliep V, Barlag K
Department of Dermatology, Heinrich-Heine University of Düsseldorf, Germany.
Exp Dermatol. 1995 Feb;4(1):46-51. doi: 10.1111/j.1600-0625.1995.tb00221.x.
There is a need in many studies on the epidermal permeability barrier for practical and reliable separation of all major fractions of human stratum corneum lipids. Various methods have been described including thin layer chromatography, high performance thin layer chromatography and iatroscan. However, none of these methods seems to be applicable for an inexpensive, rapid and reliable analysis of a large number of samples. Here, such a method for the separation and quantification of all major stratum corneum lipid fractions is presented. This method employs the one-dimensional separation of stratum corneum lipids using thin layer chromatography. The systems used herein are based upon various modifications of the solvents, solvent ratio and developing distance of each system. For quantification of chromatographed and charred lipids a Desaga densitometer is used. The system described here had been successfully applied to 550 samples of human stratum corneum lipids.
在许多关于表皮渗透屏障的研究中，需要对人角质层脂质的所有主要组分进行实用且可靠的分离。已经描述了各种方法，包括薄层色谱法、高效薄层色谱法和碘扫描法。然而，这些方法似乎都不适用于对大量样品进行廉价、快速且可靠的分析。在此，提出了一种用于分离和定量所有主要角质层脂质组分的方法。该方法采用薄层色谱法对角质层脂质进行一维分离。本文使用的系统基于每个系统的溶剂、溶剂比例和展开距离的各种改进。对于色谱分离和炭化的脂质的定量，使用Desaga密度计。这里描述的系统已成功应用于550个人角质层脂质样品。
