Serizawa S, Osawa K, Togashi K, Yamamoto A, Ito M, Hamanaka S, Otsuka F
Department of Dermatology, Niigata University School of Medicine, Japan.
J Invest Dermatol. 1992 Aug;99(2):232-6. doi: 10.1111/1523-1747.ep12650454.
To investigate the role of cholesterol sulfate (CS) as an intercellular glue or cement in the stratum corneum, we compared the relationship between CS levels and magnitude of the intercellular cohesion of the stratum corneum between the palm and the upper arm. Using a push-pull meter, the palm displayed approximately seven times the magnitude of cohesion of the stratum corneum as the upper arm (n = 11). CS and other stratum corneum lipids were extracted from the palm and the upper arm (n = 22) by a cup method and determined by our improved high-performance thin-layer chromatography (HPTLC). Despite a great difference in the magnitude of cohesion (p less than 0.01), CS levels and ratios of CS to ceramides and CS to cholesterol in the stratum corneum showed no significant differences between the palm and the upper arm. Our results suggest that differences in CS cannot account for the differences in cohesion between palm and upper arm.
为了研究硫酸胆固醇（CS）作为角质层中细胞间黏合剂或黏着剂的作用，我们比较了手掌和上臂角质层中CS水平与细胞间黏附强度之间的关系。使用推挽式测量仪，手掌角质层的黏附强度约为上臂的7倍（n = 11）。通过杯法从手掌和上臂（n = 22）中提取CS和其他角质层脂质，并采用我们改进的高效薄层色谱法（HPTLC）进行测定。尽管黏附强度差异很大（p < 0.01），但手掌和上臂角质层中的CS水平以及CS与神经酰胺的比率、CS与胆固醇的比率均无显著差异。我们的结果表明，CS的差异不能解释手掌和上臂之间黏附力的差异。
