Lucarelli F, Palchetti I, Marrazza G, Mascini M
Dipartimento di Chimica, Università degli studi di Firenze, Via della Lastruccia 3, Polo Scientifico, 50019 Firenze, Italy.
Talanta. 2002 Apr 1;56(5):949-57. doi: 10.1016/s0039-9140(01)00655-5.
Applications of a disposable electrochemical DNA biosensor to standard solutions and to real samples are reported. The DNA biosensor is assembled by immobilising the double stranded calf thymus DNA on the surface of a disposable carbon screen-printed electrode. The immobilised ds-DNA interacts with the sample for 2 min; then is washed and immersed in a clean buffer where the analytical signal (the oxidation peak area of the guanine base) is obtained by a square-wave voltammetric scan. The results were compared with some currently used toxicity tests and in particular with a commercial luminescent bacteria test, Toxalert(R)100.
报道了一种一次性电化学DNA生物传感器在标准溶液和实际样品中的应用。该DNA生物传感器是通过将双链小牛胸腺DNA固定在一次性碳丝网印刷电极表面组装而成。固定化的双链DNA与样品相互作用2分钟；然后洗涤并浸入干净的缓冲液中，通过方波伏安扫描获得分析信号（鸟嘌呤碱基的氧化峰面积）。将结果与一些目前使用的毒性测试进行了比较，特别是与一种商业发光细菌测试Toxalert(R)100进行了比较。
