磷脂酶C是否刺激部分肝切除术后制备的大鼠肝脏提取物的胸苷激酶活性？

Does phospholipase C stimulate thymide kinase activity of rat liver extracts prepared after partial hepatectomy.

作者信息

Kizer D E, Troop K, Barela T D, Holman L

出版信息

Proc Soc Exp Biol Med. 1975 Mar;148(3):838-41. doi: 10.3181/00379727-148-38644.

Abstract

Our purpose was to determine whether phospholipase C stimulated thymidine kinase activity of regenerating rat liver. We determined effects of phospholipase C upon TMP formation by rat liver extracts prepared at 0, 12, 24, 36 and 48 hr following partial hepatectomy. Data were obtained which supported these conclusions: (a) Commercial preparations of phospholipase C contained nucleoside phosphotransferase activity; (b) phospholipase C exerted no appreciable stimulatory influence upon thymidine kinase activity of regenerating rat liver; and (c), apparent stimulation of thymidine kinase was associated with linked activities of two enzymes, viz., liver extract-ATPase activity and nucleoside phosphotransferase activity.

摘要

我们的目的是确定磷脂酶C是否会刺激再生大鼠肝脏的胸苷激酶活性。我们测定了磷脂酶C对部分肝切除术后0、12、24、36和48小时制备的大鼠肝脏提取物中胸苷酸合成的影响。获得的数据支持了以下结论：(a) 市售的磷脂酶C制剂含有核苷磷酸转移酶活性；(b) 磷脂酶C对再生大鼠肝脏的胸苷激酶活性没有明显的刺激作用；(c) 胸苷激酶的明显刺激与两种酶的相关活性有关，即肝提取物ATP酶活性和核苷磷酸转移酶活性。