大鼠肝脏的胸苷激酶。磷脂酶C的激活作用及在肝脏中的亚细胞分布。
Thymidine kinase of rat liver. Activation by phospholipase C and subcellular distribution in the liver.
作者信息
Stirpe F, La Placa M
出版信息
Biochem J. 1971 Apr;122(3):347-51. doi: 10.1042/bj1220347.
Abstract
- The thymidine kinase activity of rat liver is greatly enhanced on addition of phospholipase C to the assay mixture. 2. Most of the thymidine kinase activity of the liver is recovered in the mitochondrial and in the impure ;nuclear' fractions. No activity was detected in purified nuclei prepared in high-density sucrose. 3. A substantial thymidine kinase activity could be detected, with the aid of phospholipase C, in all rat tissues examined.
摘要
- 向测定混合物中添加磷脂酶C后，大鼠肝脏的胸苷激酶活性大大增强。2. 肝脏中大部分胸苷激酶活性存在于线粒体和不纯的“核”级分中。在高密度蔗糖中制备的纯化细胞核中未检测到活性。3. 在所有检测的大鼠组织中，借助磷脂酶C可检测到大量的胸苷激酶活性。
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