Zherev S t
Eksp Med Morfol. 1975;14(1):12-6.
The author carried out on 35 cats a study on the protein content, potasium and sodium. aldolase, GOT, GPT and LDH of the uterine muscle. The animals were divided into three groups: first-15 nonpregnant cats, second-10 pregnant cats at the first half of pregnancy and third-10 pregnant cats at the second half of pregnancy. He used a piece of uterine muscle from which proteins were extracted by solutions of potasium iodide with various strength. The total protein was determined by the method of Loury, but the sarcoplasmic proteins were examined electrophoretically. Electrolytes were estimated by flame photometer. The enzyme activity was examined by the reagents of "Boehringer". There was an increase of the amount of myosin and of the enzyme active sarcoplasmic protein fraction in the myometrium of pregnant animals. Potasium was increased in the uterine muscle during pregnancy, but sodium decreased. Enxyme activity of ALD, GOT and GPT was the highest in animals from the second group, but that of LDH-in the third group.
作者对35只猫进行了一项关于子宫肌蛋白质含量、钾和钠、醛缩酶、谷草转氨酶、谷丙转氨酶和乳酸脱氢酶的研究。这些动物被分为三组:第一组15只未怀孕的猫,第二组10只妊娠前半期的怀孕猫,第三组10只妊娠后半期的怀孕猫。他使用了一块子宫肌,用不同浓度的碘化钾溶液从中提取蛋白质。总蛋白采用劳里法测定,但肌浆蛋白用电泳法检测。电解质用火焰光度计估算。酶活性用“勃林格”试剂检测。妊娠动物子宫肌中肌球蛋白量和酶活性肌浆蛋白组分增加。妊娠期间子宫肌中钾增加,但钠减少。醛缩酶、谷草转氨酶和谷丙转氨酶的酶活性在第二组动物中最高,而乳酸脱氢酶的酶活性在第三组动物中最高。