Morozova E S, Trenina G A, Pleteneva E A
Genetika. 1975;11(7):113-20.
In the course of selection of Trichoderma viride, the producer of cellulolytic enzymes, the group of mutant strains characterized by a higher level of productivity are isolate. It is shown that the isolated mutants possess a number of common but differing them from original strains characters. These include: the small size of colonies ("dwarfs"), a lower capacity to carry out some biochemical reactions, and increased development rate and a higher resistance to lethal effect of nitrosoguanidine and nitrosoethyl urea. The data obtained indicate that in the series of populations of successively isolated mutants observed the stabilization of variability of the levels of C1 enzyme synthesis takes place. It is also shown that, unlike original cultures, the populations of mutant strains are characterized by a higher variability of Cx enzyme activity levels as compared with C1.
在筛选纤维素分解酶产生菌绿色木霉的过程中,分离出了一组以较高生产力水平为特征的突变菌株。结果表明,分离出的突变体具有一些与原始菌株相同但又不同的特征。这些特征包括:菌落体积小(“侏儒型”)、进行某些生化反应的能力较低、生长速率加快以及对亚硝基胍和亚硝基乙基脲的致死效应具有更高的抗性。所获得的数据表明,在连续分离的突变体群体系列中,观察到C1酶合成水平的变异性趋于稳定。还表明,与原始培养物不同,突变菌株群体的特征是与C1相比,Cx酶活性水平具有更高的变异性。