Witkowska D, Bien M, Sobieszczański J
Department of Biotechnology and Food Microbiology, Academy of Agriculture, Wroclaw, Poland.
Microbiologia. 1989 Sep;5(2):113-9.
Trichoderma viride C-1 strain was irradiated with UV until the survival level of 0.03% was obtained. Sixteen mutants were isolated on the basis of the visible clearance zone around the colonies on the media with cellulose and glycerol. Then they were cultivated on a rotary shaker in the liquid Saunders medium supplemented with microcrystalline cellulose and comminuted sugar beet pulp. Exo-1,4-beta-glucanase, endo-1,4-beta-glucanase and beta-glucosidase were assayed in the supernatants of postcultural liquids at different time intervals of culture. The same mutants were characterized by higher biosynthesis level of exoglucanase (1.2-5.0 times) endoglucanase (1.2-2.5 times) and beta-glucosidase (1.5-1.7 times) when compared with the wild type strain.
用紫外线照射绿色木霉C-1菌株，直至获得0.03%的存活水平。根据在含有纤维素和甘油的培养基上菌落周围可见的透明圈，分离出16个突变体。然后将它们在旋转摇床上培养于添加了微晶纤维素和粉碎甜菜粕的液体桑德斯培养基中。在培养的不同时间间隔，测定培养后液体上清液中的外切-1,4-β-葡聚糖酶、内切-1,4-β-葡聚糖酶和β-葡萄糖苷酶。与野生型菌株相比，相同的突变体在外切葡聚糖酶（1.2 - 5.0倍）、内切葡聚糖酶（1.2 - 2.5倍）和β-葡萄糖苷酶（1.5 - 1.7倍）的生物合成水平上更高。
