Ostrikova N A, Konovalov S A
Mikrobiologiia. 1980 Jul-Aug;49(4):540-5.
A mutant strain of Trichoderma viride producing cellulase has been obtained as a result of three-stage selection using chemical and physical factors. New variants with modified characteristics appeared after the action of nitroso compounds on a suspension of conidia at the first stage of selection and the action of temperature at the second stage. The new characteristics were intensified and became stable at the third stage of selection. The experimental mutagenesis yielded an active an active mutant of Trichoderma viride 44 which synthesized cellulase (up to 22 units/ml) four times better than the parent culture Trichoderma viride F-57.
通过使用化学和物理因素进行三阶段筛选，获得了一种产生纤维素酶的绿色木霉突变菌株。在筛选的第一阶段，亚硝基化合物作用于分生孢子悬液，第二阶段温度作用后，出现了具有改良特性的新变体。在筛选的第三阶段，新特性得到强化并变得稳定。实验诱变产生了一种活性的绿色木霉44活性突变体，其合成纤维素酶的能力（高达22单位/毫升）比亲本培养物绿色木霉F-57高出四倍。
