Fossi M C, Casini S, Marsili L, Neri G, Mori G, Ancora S, Moscatelli A, Ausili A, Notarbartolo-di-Sciara G
Department of Animal Biology and Marine Ecology, University of Messina, Italy.
Mar Environ Res. 2002 Sep-Dec;54(3-5):667-71. doi: 10.1016/s0141-1136(02)00149-6.
The hypothesis that Mediterranean top predator species, such as large pelagic fish, are potentially at risk due to endocrine disrupting chemicals (EDCs), is investigated. The potential estrogenic effects of PHAHs in three fish species of commercial interest, the top predators bluefin tuna (Thunnus thynnus thynnus), swordfish (Xiphias gladius), and Mediterranean spearfish (Tetrapturus belone), were investigated using vitellogenin (Vtg), zona radiata proteins (Zrp) and mixed function oxidases (EROD, BPMO) as diagnostic tools. High induction of Vtg and Zrp was detected by western blot and ELISA techniques in adult males of X. gladius and T. thynnus thynnus, suggesting that these species are at high toxicological risk in the Mediterranean sea. Comparison of BPMO and EROD activities in the three species indicated, both in male and female, much higher MFO activity in bluefin tuna. This data suggests high exposure of this species to lipophilic xenobiotic contaminants in the Mediterranean environment.
本研究调查了地中海顶级捕食者物种，如大型远洋鱼类，因内分泌干扰化学物质（EDC）而面临潜在风险这一假说。使用卵黄蛋白原（Vtg）、放射带蛋白（Zrp）和混合功能氧化酶（EROD、BPMO）作为诊断工具，研究了多环芳烃（PHAH）对三种具有商业价值的鱼类（顶级捕食者蓝鳍金枪鱼（Thunnus thynnus thynnus）、剑鱼（Xiphias gladius）和地中海旗鱼（Tetrapturus belone））的潜在雌激素效应。通过蛋白质免疫印迹法和酶联免疫吸附测定技术，在剑鱼和大西洋蓝鳍金枪鱼的成年雄性个体中检测到了Vtg和Zrp的高诱导，这表明这些物种在地中海海域面临着高毒理学风险。对这三种鱼类的BPMO和EROD活性进行比较后发现，无论雄性还是雌性，蓝鳍金枪鱼的混合功能氧化酶活性都要高得多。该数据表明，在地中海环境中，该物种高度暴露于亲脂性外源性污染物。
