阿拉磷作为从临床样本中分离沙门氏菌的选择剂。
Alafosfalin as a selective agent for isolation of salmonella from clinical samples.
作者信息
Perry J D, Riley G, Gould F K, Perez J M, Boissier E, Ouedraogo R T, Freydière A M
机构信息
Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom.
出版信息
J Clin Microbiol. 2002 Nov;40(11):3913-6. doi: 10.1128/JCM.40.11.3913-3916.2002.
The selectivity of a range of culture media for the detection of Salmonella was assessed using 435 strains of gram-negative bacteria. These media showed limited ability to inhibit non-Salmonella strains found in stool samples. We report the evaluation of alafosfalin as a selective agent for isolation of Salmonella from stool samples. Susceptibility studies with this agent showed that non-typhi Salmonella strains were relatively resistant (mean MIC, 10.2 mg/liter) compared to many coliforms including Escherichia coli (mean MIC, 0.7 mg/liter). A chromogenic medium, ABC medium, was modified to incorporate alafosfalin and was compared with standard ABC medium and Hektoen enteric agar for the isolation of Salmonella from 1,000 stool samples. On direct culture, modified ABC medium showed higher recovery of Salmonella (53.6%) compared with either ABC medium (35.7%) or Hektoen enteric agar (48.2%). We conclude that alafosfalin is a useful selective agent for the isolation of Salmonella from stool samples.
使用435株革兰氏阴性菌评估了一系列用于检测沙门氏菌的培养基的选择性。这些培养基抑制粪便样本中发现的非沙门氏菌菌株的能力有限。我们报告了对阿拉磷作为从粪便样本中分离沙门氏菌的选择性试剂的评估。对该试剂的敏感性研究表明，与包括大肠杆菌（平均最低抑菌浓度，0.7毫克/升）在内的许多大肠菌群相比，非伤寒沙门氏菌菌株相对耐药（平均最低抑菌浓度，10.2毫克/升）。一种显色培养基ABC培养基被改良以加入阿拉磷，并与标准ABC培养基和赫氏肠道琼脂用于从1000份粪便样本中分离沙门氏菌进行比较。直接培养时，改良ABC培养基显示出比ABC培养基（35.7%）或赫氏肠道琼脂（48.2%）更高的沙门氏菌回收率（53.6%）。我们得出结论，阿拉磷是从粪便样本中分离沙门氏菌的一种有用的选择性试剂。
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