两种新型显色培养基用于粪便中沙门氏菌检测的评估
Evaluation of two new chromogenic media for detection of Salmonella in stools.
作者信息
Monnery I, Freydiere A M, Baron C, Rousset A M, Tigaud S, Boude-Chevalier M, de Montclos H, Gille Y
机构信息
Laboratoire de Microbiologie, Hôpital de l'Antiquaille, Institut Pasteur de Lyon, France.
出版信息
Eur J Clin Microbiol Infect Dis. 1994 Mar;13(3):257-61. doi: 10.1007/BF01974547.
A clinical collaborative study was conducted to compare two new chromogenic agar media, Rambach agar and the Salmonella Detection and Identification Medium (SMID) (bioMérieux, France), with two conventional media, Salmonella-Shigella agar and Hektoen agar. Thirty-nine Salmonella strains involving 14 serotypes were isolated from 1,454 stool specimens. After enrichment in a selective broth, 100% sensitivity was obtained with each medium. The SMID and Rambach agars are considerably more specific than the conventional media. Although SMID agar detects all Salmonella serotypes, it is not as specific as Rambach agar, which requires a complementary test (C8 esterase test) to detect all serotypes.
进行了一项临床协作研究，以比较两种新型显色琼脂培养基（兰巴赫琼脂和沙门氏菌检测与鉴定培养基（SMID），法国生物梅里埃公司）与两种传统培养基（沙门氏菌-志贺氏菌琼脂和赫克托恩琼脂）。从1454份粪便标本中分离出涉及14种血清型的39株沙门氏菌菌株。在选择性肉汤中增菌后，每种培养基的敏感性均达到100%。SMID琼脂和兰巴赫琼脂比传统培养基的特异性高得多。虽然SMID琼脂能检测所有沙门氏菌血清型，但它不如兰巴赫琼脂特异性高，兰巴赫琼脂需要进行一项补充试验（C8酯酶试验）来检测所有血清型。
Zotero 插件