Gamboa Liliana, Camou Teresa, Hortal María, Castañeda Elizabeth
Grupo de Microbiologia, Instituto Nacional de Salud, Bogotá, Colombia.
J Clin Microbiol. 2002 Nov;40(11):3942-50. doi: 10.1128/JCM.40.11.3942-3950.2002.
Streptococcus pneumoniae serotype 5 is the third most common capsular type causing invasive diseases in children younger than 5 years in Latin America. Preliminary data on Colombian serotype 5 isolates indicated a common clonal origin associated with resistance to tetracycline (TET) and chloramphenicol (CHL). We studied 172 S. pneumoniae serotype 5 invasive isolates from Argentina, Brazil, Colombia, Guatemala, Mexico, and Uruguay and confirmed the presence of the Colombia(5)-19 clone throughout Latin America. Fifteen subtypes of a pulsed-field gel electrophoresis pattern and 4 electrophoretic types (ET) were obtained. Most of the isolates from different geographical regions belonged to pattern A (34.3%), subtype A5 (41.9%), and ET1 (91.1%). The A pattern (n = 59) was resistant to TET and had variable resistance to CHL; it was present in Brazil (10.2%), Colombia (78%), Guatemala (8.5%), and Mexico (3.4%). Subtype A5 with variable susceptibility to TET and sensitive to CHL was found in Argentina (29.2%), Mexico (8.3%), and Uruguay (62.5%). Subtypes A1-A4, A7-A8, and A9-A11 (closely related to A) also shared ET1, while subtype A6 was assigned to ET1, ET2, and ET3. Eleven subtypes (n = 21) were found to be specific for one country each. In summary, the S. pneumoniae serotype 5 isolates from Latin American are genetically closely related but show different patterns of antibiotic resistance, probably as a result of horizontal transfer.
肺炎链球菌5型是拉丁美洲5岁以下儿童侵袭性疾病的第三大常见荚膜型。关于哥伦比亚5型分离株的初步数据表明，其存在与四环素（TET）和氯霉素（CHL）耐药性相关的共同克隆起源。我们研究了来自阿根廷、巴西、哥伦比亚、危地马拉、墨西哥和乌拉圭的172株肺炎链球菌5型侵袭性分离株，证实了整个拉丁美洲都存在哥伦比亚（5）-19克隆。获得了脉冲场凝胶电泳图谱的15个亚型和4种电泳类型（ET）。来自不同地理区域的大多数分离株属于A模式（34.3%）、A5亚型（41.9%）和ET1（91.1%）。A模式（n = 59）对TET耐药，对CHL耐药性各异；它存在于巴西（10.2%）、哥伦比亚（78%）、危地马拉（8.5%）和墨西哥（3.4%）。在阿根廷（29.2%）、墨西哥（8.3%）和乌拉圭（62.5%）发现了对TET敏感性各异且对CHL敏感的A5亚型。A1 - A4、A7 - A8和A9 - A11亚型（与A密切相关）也共享ET1，而A6亚型被归为ET1、ET2和ET3。发现有11个亚型（n = 21）分别是每个国家特有的。总之，拉丁美洲的肺炎链球菌5型分离株在基因上密切相关，但显示出不同的抗生素耐药模式，这可能是水平转移的结果。
