Tamayo M, Sá-Leão R, Santos Sanches I, Castañeda E, de Lencastre H
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
J Clin Microbiol. 1999 Jul;37(7):2337-42. doi: 10.1128/JCM.37.7.2337-2342.1999.
A national surveillance conducted in Colombia between 1994 and 1996 identified serotype 5 Streptococcus pneumoniae as the second most frequent cause of invasive disease in children younger than 5 years of age. All 43 serotype 5 isolates collected during this period were shown to be susceptible to penicillin, erythromycin, cefotaxime, and vancomycin, but most (38 of 43, or 88%) were highly resistant to chloramphenicol. In order to clarify a possible genetic relatedness among these isolates, additional microbiological and molecular characterizations were performed. Most (40 of 43, or 93%) of the isolates were found to be resistant to tetracycline. Pulsed-field gel electrophoresis (PFGE) patterns of chromosomal DNAs revealed that all the 43 isolates were closely related and that 38 of the 43 isolates were representatives of a "Colombian clone" of S. pneumoniae isolates which were recovered throughout the 3-year surveillance period from patients in 13 hospitals located in five Colombian cities. Isolates belonging to this Colombian clone were resistant to chloramphenicol and tetracycline, hybridized with the cat and tetM DNA probes in the same 340-kb SmaI fragment, and had identical PFGE patterns after both SmaI and ApaI digestions.
1994年至1996年在哥伦比亚进行的一项全国性监测发现，5型肺炎链球菌是5岁以下儿童侵袭性疾病的第二大常见病因。在此期间收集的所有43株5型分离株均对青霉素、红霉素、头孢噻肟和万古霉素敏感，但大多数（43株中的38株，即88%）对氯霉素高度耐药。为了阐明这些分离株之间可能存在的遗传相关性，进行了额外的微生物学和分子特征分析。大多数（43株中的40株，即93%）分离株被发现对四环素耐药。染色体DNA的脉冲场凝胶电泳（PFGE）图谱显示，所有43株分离株密切相关，43株中的38株是肺炎链球菌“哥伦比亚克隆”的代表，这些分离株是在为期3年的监测期间从哥伦比亚五个城市13家医院的患者中分离得到的。属于该哥伦比亚克隆的分离株对氯霉素和四环素耐药，在同一个340 kb的SmaI片段中与cat和tetM DNA探针杂交，并且在SmaI和ApaI消化后具有相同的PFGE图谱。
