Jayasankar Vidya, Tsutsui Naoaki, Jasmani Safiah, Saido-Sakanaka Hisako, Yang Wei-Jun, Okuno Atsuro, Tran Thi Thanh Hien, Aida Katsumi, Wilder Marcy N
Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan.
J Exp Zool. 2002 Dec 1;293(7):675-82. doi: 10.1002/jez.10167.
The dynamics of vitellogenin mRNA expression during ovarian maturation in Macrobrachium rosenbergii were examined by measuring hemolymph vitellogenin (Vg) levels and Vg mRNA expression in the hepatopancreas and ovary at differing reproductive stages in both intact and eyestalk ablated animals. Vg mRNA was quantified using real-time RT-PCR and hemolymph Vg was measured by enzyme immunoassay. In intact animals, Vg mRNA levels in the hepatopancreas and hemolymph Vg levels showed a gradual increase during the molt cycle concomitant with increasing gonadosomatic index (GSI), with Vg levels decreasing prior to ecdysis although GSI continued to increase. Eyestalk ablation was seen to accelerate Vg synthesis as well as ovarian maturation, although it did not alter the overall pattern of Vg expression. Vg mRNA expression was negligible in the ovary of both intact and eyestalk ablated animals, confirming that the hepatopancreas is the principal site of Vg synthesis in M. rosenbergii with the ovary being only a minor contributor. This study has shown that Vg synthesis is correlated to ovarian maturation and the molt cycle in M. rosenbergii.
通过测量罗氏沼虾在完整和眼柄切除动物不同生殖阶段的血淋巴卵黄蛋白原（Vg）水平以及肝胰腺和卵巢中Vg mRNA的表达，研究了罗氏沼虾卵巢成熟过程中卵黄蛋白原mRNA表达的动态变化。使用实时RT-PCR对Vg mRNA进行定量，并通过酶免疫测定法测量血淋巴Vg。在完整动物中，肝胰腺中的Vg mRNA水平和血淋巴Vg水平在蜕皮周期中随着性腺指数（GSI）的增加而逐渐升高，尽管GSI持续增加，但在蜕皮前Vg水平下降。眼柄切除可加速Vg合成以及卵巢成熟，尽管它并未改变Vg表达的总体模式。在完整和眼柄切除动物的卵巢中，Vg mRNA表达均可忽略不计，这证实肝胰腺是罗氏沼虾中Vg合成的主要部位，而卵巢只是次要贡献者。这项研究表明，罗氏沼虾中Vg合成与卵巢成熟和蜕皮周期相关。
