Chen M, Fan M Y, Bi D Z, Zhang J Z, Huang Y P
Department of Rickettsiology, Chinese Academy of Preventive Medicine, Beijing, P.R. of China.
Acta Virol. 1998 Feb;42(1):61-4.
The primers Rr 190.70p and Rr 190.602n were used to detect spotted fever group (SFG) rickettsiae by polymerase chain reaction (PCR) in ticks and small mammals collected in three different regions of China. The obtained results indicated that specific DNA fragments of SFG rickettsiae were amplified from Dermacentor silvarum, D. sinicus, D. auratus, Haemaphysalis concinna, H. wellingtoni, H. yeni, Apodemus agrarius, Microtus fortis. Clethrionomys rufocanus, Ondatra zibethica, Rattus flavipectus and hedgehog. The PCR product were digested with restriction endonucleases PstI and RsaI and the obtained electrophoretic profiles were compared with those of the prototype strains of SFG rickettsiae by the restriction fragment length polymorphism (RFLP) technique. The comparisons showed that the profiles were identical to those of Rickettsia sibirica. In addition, three new isolates of R. sibirica were obtained from H. yeni, D. sinicus and hedgehog, and designated NH-95, BJ-95 and BHJ-95, respectively. These results not only demonstrated a horizontal transmission of the rickettsiae between ticks and hosts but also suggested that R. sibirica is widely distributed in China and its hosts and vectors are various, all that indicating the existence of natural foci of North Asia tick-borne spotted fever specific to China.
利用引物Rr 190.70p和Rr 190.602n，通过聚合酶链反应（PCR）对采自中国三个不同地区的蜱类和小型哺乳动物进行检测，以确定斑点热群（SFG）立克次体。所得结果表明，从森林革蜱、中华革蜱、金泽革蜱、嗜群血蜱、威氏血蜱、叶氏血蜱、黑线姬鼠、东方田鼠、棕背䶄、麝鼠、黄胸鼠和刺猬中扩增出了SFG立克次体的特异性DNA片段。用限制性内切酶PstI和RsaI对PCR产物进行消化，并通过限制性片段长度多态性（RFLP）技术将所得电泳图谱与SFG立克次体原型菌株的图谱进行比较。比较结果显示，这些图谱与西伯利亚立克次体的图谱相同。此外，从叶氏血蜱、中华革蜱和刺猬中获得了三株新的西伯利亚立克次体分离株，分别命名为NH-95、BJ-95和BHJ-95。这些结果不仅证明了立克次体在蜱类和宿主之间的水平传播，还表明西伯利亚立克次体在中国广泛分布，其宿主和传播媒介多种多样，所有这些都表明中国存在北亚蜱传斑点热的自然疫源地。
