Timofeeva M Ia, Eĭsner G I, Kupriianova N S
Mol Biol (Mosk). 1975 Jan-Feb;9(1):126-33.
DNA renaturation kinetics was measured for the genomes of normal (spleen) and malignant (plasmacytoma) mouse tissues and for DNA from liver, sperm and developing embryos of the loach. It has been shown that the measuring of DNA renaturation kinetics makes it possible to reveal differences in the content of certain fractions of the repetitions in the genomes of different species. Moreover, differences in the distribution of the repetitions between hetero- and euchromatine can be identified. Loach embryo DNA (blastula stage) was shown to contain larger amount of the fraction renaturing at Cot less than 10(-2) as compared to liver and sperm DNAs (by 5%). An enrichment with respect to the intermediate repetitions (10(-2) less than Cot less than 10(2)) was found in the mouse plasmacytoma DNA as compared to the spleen DNA. The nature of these distinctions is discussed.
对正常（脾脏）和恶性（浆细胞瘤）小鼠组织的基因组以及泥鳅肝脏、精子和发育中胚胎的DNA进行了DNA复性动力学测定。结果表明，通过测定DNA复性动力学能够揭示不同物种基因组中某些重复序列组分含量的差异。此外，还能确定异染色质和常染色质之间重复序列分布的差异。与肝脏和精子DNA相比，泥鳅胚胎DNA（囊胚期）显示出在Cot小于10^(-2)时复性的组分含量更高（高出5%）。与脾脏DNA相比，在小鼠浆细胞瘤DNA中发现了中间重复序列（10^(-2) ＜ Cot ＜ 10^(2)）的富集。对这些差异的本质进行了讨论。
