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[鲑鱼样鱼类两种结构中基因组结构的种群内差异]

[Intrapopulational differences of genome structures in two structures of salmon-like fishes].

作者信息

Kosiuk G N, Borkhsenius S N

出版信息

Mol Biol (Mosk). 1981 May-Jun;15(3):547-53.

PMID:6789144
Abstract

The natural populations of salmon-like catadromous fishes usually include several percents of residual (dwarf) forms, which are 10-15 times smaller than normal forms. A comparative investigation of normal and residual forms in two species: Oncorhynchus nerka and Salvelinus malma (Salmoniformes order) was made by means of DNA molecular hybridization technique. The essential differences in reassociation kinetics was detected in DNA from normal and residual forms of both species. The genome sizes (kinetic complexity) of normal and residual forms are approximately the same. But some families of repetitive nucleotide sequences are represented by considerably different amount of copies. Intrapopulational differences of genome structures of normal and residual forms in both species seem to be more rough in respect to reassociation kinetic than corresponding interspecies differences between malma and nerka. Comparative analyses and reassociation kinetics for long and shrt DNA fragments imply "xenopus type" of nucleotide sequence organization in both species which is common for the majority of animal and plant genomes investigated.

摘要

鲑形溯河产卵鱼类的自然种群通常包含百分之几的残留(侏儒)形态,其体型比正常形态小10至15倍。借助DNA分子杂交技术,对两种鱼类:红大麻哈鱼和马苏大麻哈鱼(鲑形目)的正常形态和残留形态进行了比较研究。在这两个物种的正常形态和残留形态的DNA中检测到了重新结合动力学的本质差异。正常形态和残留形态的基因组大小(动力学复杂度)大致相同。但是一些重复核苷酸序列家族的拷贝数存在显著差异。就重新结合动力学而言,这两个物种中正常形态和残留形态的基因组结构的种群内差异似乎比马苏大麻哈鱼和红大麻哈鱼之间相应的种间差异更为显著。对长DNA片段和短DNA片段的比较分析及重新结合动力学表明,这两个物种的核苷酸序列组织为“非洲爪蟾型”,这在大多数已研究的动植物基因组中很常见。

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