Kupriianova N S, Timofeeva M I, Baev A A
Mol Biol (Mosk). 1976 Mar-Apr;10(2):412-22.
About 5% of the loach (Misgurnus fossillis L.) DNA reassociates at Cot values virtually equal to zero. 50% of the reassociate are resistant to nuclease S1 treatment and reveal the properties of double-stranded structure when chromatographed on hydroxyapatite. Some proofs of palindromic (hair-pin) nature of this fraction have been obtained. An introduction of nicked scissions into the palindromic DNA by pancreatic DNAse treatment under pessimal conditions made it possible to investigated reassociation kinetics of the nucleotide sequences forming palindromes. Two different types of nucleotide sequences appeared to exist in the palindromic fraction with repetition frequencies (ni) equal to 3 X 10(2) and about 1. Homologies were revealed between these sequences and the fraction of corresponding repetition frequency of the main part of the genome. Adjacent sequences contain repetitive regions with ni equal to 10(5) and 5 X 10(3). On the basis of the data obtained some conclusions were made about the distribution of usual and inverted repetitions in the loach genome.
约5%的泥鳅（Misgurnus fossillis L.）DNA在Cot值几乎等于零时重新缔合。重新缔合的DNA中有50%对核酸酶S1处理具有抗性，并且在羟基磷灰石上进行色谱分析时显示出双链结构的特性。已经获得了该部分具有回文（发夹）性质的一些证据。在最不利的条件下，通过胰脱氧核糖核酸酶处理在回文DNA中引入切口，使得研究形成回文的核苷酸序列的重新缔合动力学成为可能。回文部分似乎存在两种不同类型的核苷酸序列，其重复频率（ni）分别等于3×10²和约1。这些序列与基因组主要部分相应重复频率的部分之间显示出同源性。相邻序列包含重复频率ni分别等于10⁵和5×10³的重复区域。根据所获得的数据，对泥鳅基因组中正向和反向重复的分布得出了一些结论。
