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硫化氢供体对猪卵丘-卵母细胞复合体减数分裂和卵丘扩展的双重作用。

Dual effects of hydrogen sulfide donor on meiosis and cumulus expansion of porcine cumulus-oocyte complexes.

作者信息

Nevoral Jan, Petr Jaroslav, Gelaude Armance, Bodart Jean-Francois, Kucerova-Chrpova Veronika, Sedmikova Marketa, Krejcova Tereza, Kolbabova Tereza, Dvorakova Marketa, Vyskocilova Alena, Weingartova Ivona, Krivohlavkova Lenka, Zalmanova Tereza, Jilek Frantisek

机构信息

Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague, Czech Republic.

Research Institute of Animal Production, Prague, Czech Republic.

出版信息

PLoS One. 2014 Jul 1;9(7):e99613. doi: 10.1371/journal.pone.0099613. eCollection 2014.

DOI:10.1371/journal.pone.0099613
PMID:24984032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4077697/
Abstract

Hydrogen sulfide (H2S) has been revealed to be a signal molecule with second messenger action in the somatic cells of many tissues, including the reproductive tract. The aim of this study was to address how exogenous H2S acts on the meiotic maturation of porcine oocytes, including key maturation factors such as MPF and MAPK, and cumulus expansion intensity of cumulus-oocyte complexes. We observed that the H2S donor, Na2S, accelerated oocyte in vitro maturation in a dose-dependent manner, following an increase of MPF activity around germinal vesicle breakdown. Concurrently, the H2S donor affected cumulus expansion, monitored by hyaluronic acid production. Our results suggest that the H2S donor influences oocyte maturation and thus also participates in the regulation of cumulus expansion. The exogenous H2S donor apparently affects key signal pathways of oocyte maturation and cumulus expansion, resulting in faster oocyte maturation with little need of cumulus expansion.

摘要

硫化氢（H₂S）已被证实是一种在包括生殖道在内的许多组织的体细胞中具有第二信使作用的信号分子。本研究的目的是探讨外源性H₂S如何作用于猪卵母细胞的减数分裂成熟，包括关键的成熟因子如MPF和MAPK，以及卵丘-卵母细胞复合体的卵丘扩展强度。我们观察到，H₂S供体Na₂S以剂量依赖的方式加速卵母细胞的体外成熟，在生发泡破裂前后MPF活性增加。同时，H₂S供体通过透明质酸产生监测影响卵丘扩展。我们的结果表明，H₂S供体影响卵母细胞成熟，从而也参与卵丘扩展的调节。外源性H₂S供体明显影响卵母细胞成熟和卵丘扩展的关键信号通路，导致卵母细胞更快成熟，且几乎不需要卵丘扩展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/7be0e1c0e7e9/pone.0099613.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/9eb8f5a9a00e/pone.0099613.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/4708d3f55ec2/pone.0099613.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/7be0e1c0e7e9/pone.0099613.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/9eb8f5a9a00e/pone.0099613.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/dc1f757665e3/pone.0099613.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/bdcea493aca7/pone.0099613.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/4708d3f55ec2/pone.0099613.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2eb1/4077697/7be0e1c0e7e9/pone.0099613.g005.jpg

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