Bozkurt G, Memis D, Karabogaz G, Pamukcu Z, Ture M, Karamanlioglu B, Gunday I, Algunes C
Department of Medical Genetics, Medical Faculty, Trakya University, Edime, Turkey.
Anaesth Intensive Care. 2002 Oct;30(5):597-602. doi: 10.1177/0310057X0203000509.
The possibility of a potential mutagenic or carcinogenic action of chronic exposure to low concentrations of inhalational anaesthetics has been previously studied, with conflicting results. The purpose of this study was to assess whether occupational exposure to waste anaesthetic gases increases genotoxic risk. We examined peripheral lymphocytes from anaesthetists for both sister chromatid exchange (SCE) and for cells with high-frequency SCEs (HFCs).
A group of 16 non-smoking anaesthetists with occupational exposure to anaesthetic gases and a sex- and age-matched group matched 16 non-smoking matched physicians without occupational exposure to anaesthetic gases were studied. The participants were also selected on the basis of similar responses to a questionnaire assessing risk of genotoxicity relating to other aspects of life.
SCEs, and HFC percentages obtained from the exposed anaesthetists (6.6+/-2.4 and 12.2+/-15.9) were greater but not statistically significantly so than in the reference group (5.2+/-1.6 and 5.9+/-10.0).
This study does not support the existence of an association between occupational exposure to waste anaesthetic gases and an increase in SCEs in lymphocytes. The nature of our anaesthesia practice suggests exposure was likely to be low. It should be noted that some anaesthetic gases produce lesions that can be efficiently repaired in mitogen-stimulated lymphocytes in vitro but not in circulating lymphocytes.
既往曾研究过长期暴露于低浓度吸入性麻醉剂可能产生的诱变或致癌作用，结果相互矛盾。本研究的目的是评估职业性接触废弃麻醉气体是否会增加遗传毒性风险。我们检测了麻醉医生外周血淋巴细胞的姐妹染色单体交换（SCE）情况以及具有高频SCE（HFC）的细胞情况。
对一组16名有职业性接触麻醉气体的非吸烟麻醉医生，以及一组16名年龄和性别匹配、无职业性接触麻醉气体的非吸烟医生进行了研究。参与者还根据对一份评估与生活其他方面相关的遗传毒性风险问卷的类似回答进行选择。
接触麻醉气体的麻醉医生的SCE和HFC百分比（分别为6.6±2.4和12.2±15.9）高于对照组（分别为5.2±1.6和5.9±10.0），但差异无统计学意义。
本研究不支持职业性接触废弃麻醉气体与淋巴细胞SCE增加之间存在关联。我们麻醉操作的性质表明接触程度可能较低。应当注意的是，一些麻醉气体产生的损伤在体外有丝分裂原刺激的淋巴细胞中能够有效修复，但在循环淋巴细胞中则不能。
