Barbisan L F, Miyamoto M, Scolastici C, Salvadori D M F, Ribeiro L R, Eira A F, de Camargo João Lauro Viana
Departamento de Morfologia, Instituto de Biologia, UNESP, Botucatu 18618-000, SP, Brazil.
J Ethnopharmacol. 2002 Nov;83(1-2):25-32. doi: 10.1016/s0378-8741(02)00171-x.
The modifying potential of prior administration of an aqueous extract of the mushroom Agaricus blazei Murrill (Agaricaceae) (Ab) on hepatotoxicity induced by different doses of diethylnitrosamine (DEN) in male Wistar rats was evaluated. During 2 weeks, animals of groups G3 (Ab+DEN(50)), G5 (Ab+DEN(100)), G7 (Ab+DEN(200)), and G8 (Ab-treated) were treated with the A. blazei through drinking water. After this period, groups G2 (DEN(50)), G3 (Ab+DEN(50)), G4 (DEN(100)) G5 (Ab+DEN(100)), G6 (DEN(200)), and G7 (Ab+DEN(200)) were given a single i.p. injection of 50, 100 and 200 mg/kg of DEN, respectively, while groups G1 (non-treated) and G8 (Ab-treated) were treated with 0.9% NaCl only. All animals were killed 48 h after DEN or NaCl treatments. The hepatocyte replication rate was estimated by the index of the proliferating cell nuclear antigen (PCNA) positive hepatocytes and the appearance of putative preneoplastic hepatocytes through expression of the enzyme glutathione S-transferase placental form (GST-P). After DEN-treatment, ALT levels, PCNA labeling index, and the number of GST-P positive hepatocytes were lower in rats that received A. blazei treatment and were exposed to 100 mg/kg of DEN. Our findings suggest that previous treatment with A. blazei exerts a hepatoprotective effect on both liver toxicity and hepatocarcinogenesis process induced by a moderately toxic dose of DEN.
评估了预先给予姬松茸（蘑菇科）水提取物（Ab）对雄性Wistar大鼠不同剂量二乙基亚硝胺（DEN）诱导的肝毒性的调节潜力。在2周内，G3组（Ab + DEN（50））、G5组（Ab + DEN（100））、G7组（Ab + DEN（200））和G8组（Ab处理组）的动物通过饮用水给予姬松茸。在此期间后，G2组（DEN（50））、G3组（Ab + DEN（50））、G4组（DEN（100））、G5组（Ab + DEN（100））、G6组（DEN（200））和G7组（Ab + DEN（200））分别腹腔注射50、100和200 mg/kg的DEN，而G1组（未处理组）和G8组（Ab处理组）仅用0.9%氯化钠处理。在DEN或氯化钠处理后48小时处死所有动物。通过增殖细胞核抗原（PCNA）阳性肝细胞指数和通过谷胱甘肽S - 转移酶胎盘形式（GST - P）酶的表达来评估假定的癌前肝细胞的出现情况，以此估计肝细胞复制率。在DEN处理后，接受姬松茸处理并暴露于100 mg/kg DEN的大鼠的ALT水平、PCNA标记指数和GST - P阳性肝细胞数量较低。我们的研究结果表明，预先用姬松茸处理对中等毒性剂量DEN诱导的肝毒性和肝癌发生过程均具有肝保护作用。
