de Santi-Rampazzo Ana Paula, Schoffen João Paulo Ferreira, Cirilo Carla Possani, Zapater Mariana Cristina Vicente Umada, Vicentini Fernando Augusto, Soares Andréia Assunção, Peralta Rosane Marina, Bracht Adelar, Buttow Nilza Cristina, Natali Maria Raquel Marçal
Department of Morphological Sciences, State University of Maringá (UEM), 87020-900 Maringá, PR, Brazil.
Center of Biological Sciences, State University of the North of Paraná (UENP), Bandeirantes, PR, Brazil.
Evid Based Complement Alternat Med. 2015;2015:287153. doi: 10.1155/2015/287153. Epub 2015 Apr 16.
This study evaluated the effects of the supplementation with aqueous extract of Agaricus blazei Murrill (ABM) on biometric and blood parameters and quantitative morphology of the myenteric plexus and jejunal wall in aging Wistar rats. The animals were euthanized at 7 (C7), 12 (C12 and CA12), and 23 months of age (C23 and CA23). The CA12 and CA23 groups received a daily dose of ABM extract (26 mg/animal) via gavage, beginning at 7 months of age. A reduction in food intake was observed with aging, with increases in the Lee index, retroperitoneal fat, intestinal length, and levels of total cholesterol and total proteins. Aging led to a reduction of the total wall thickness, mucosa tunic, villus height, crypt depth, and number of goblet cells. In the myenteric plexus, aging quantitatively decreased the population of HuC/D(+) neuronal and S100(+) glial cells, with maintenance of the nNOS(+) nitrergic subpopulation and increase in the cell body area of these populations. Supplementation with the ABM extract preserved the myenteric plexus in old animals, in which no differences were detected in the density and cell body profile of neurons and glial cells in the CA12 and CA23 groups, compared with C7 group. The supplementation with the aqueous extract of ABM efficiently maintained myenteric plexus homeostasis, which positively influenced the physiology and prevented the death of the neurons and glial cells.
本研究评估了添加巴西蘑菇水提取物（ABM）对衰老Wistar大鼠的生物特征和血液参数以及肌间神经丛和空肠壁定量形态的影响。这些动物在7个月（C7）、12个月（C12和CA12）和23个月龄（C23和CA23）时被安乐死。CA12和CA23组从7个月龄开始每天通过灌胃给予ABM提取物（26毫克/只动物）。随着年龄增长，观察到食物摄入量减少，Lee指数、腹膜后脂肪、肠长度以及总胆固醇和总蛋白水平增加。衰老导致肠壁总厚度、黏膜层、绒毛高度、隐窝深度和杯状细胞数量减少。在肌间神经丛中，衰老使HuC/D(+)神经元和S100(+)神经胶质细胞数量定量减少，nNOS(+)一氧化氮能亚群保持不变，且这些细胞群体的细胞体面积增加。补充ABM提取物可使老年动物的肌间神经丛得以保存，与C7组相比，CA12和CA23组神经元和神经胶质细胞的密度和细胞体形态未检测到差异。补充ABM水提取物可有效维持肌间神经丛的稳态，对生理功能产生积极影响，并防止神经元和神经胶质细胞死亡。
