Tsunemi Yuichiro, Saeki Hidehisa, Nakamura Koichiro, Sekiya Takashi, Hirai Koichi, Fujita Hideki, Asano Noriko, Kishimoto Megumi, Tanida Yuka, Kakinuma Takashi, Mitsui Hiroshi, Tada Yayoi, Wakugawa Motoshi, Torii Hideshi, Komine Mayumi, Asahina Akihiko, Tamaki Kunihiko
Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
J Dermatol Sci. 2002 Nov;30(2):161-6. doi: 10.1016/s0923-1811(02)00072-5.
Interleukin-12 (IL-12) is believed to play an important role in inducing Th1-type cytokine profiles. Atopic dermatitis (AD) and psoriasis vulgaris (PsV) are considered to be Th2 and Th1 type disease, respectively. The IL-12 p40 subunit gene (IL12B) is located at chromosome 5q31-33 and linkage findings of AD on 5q31 were reported. Recently single nucleotide polymorphism (SNP) (1188A/C) of IL12B has been reported. In function, it has been reported that this SNP is associated with IL12B mRNA expression levels. To learn whether this SNP is associated with susceptibility to AD or PsV, we investigated the genotype and allele frequencies of the SNP in AD patients, in PsV patients and in controls, examining 164 AD patients, 143 PsV patients and 100 healthy individuals in Japanese population. Genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method. The A allele was decreased in AD patients (40.9%, p = 0.031) and increased in PsV patients (60.1%, p = 0.035) compared with controls (50.5%). This suggests that IL12B SNP is associated with susceptibility to AD and PsV, presumably by affecting the Th1/Th2 balance.
白细胞介素-12（IL-12）被认为在诱导Th1型细胞因子谱方面发挥重要作用。特应性皮炎（AD）和寻常型银屑病（PsV）分别被认为是Th2型和Th1型疾病。IL-12 p40亚基基因（IL12B）位于5号染色体的5q31-33区域，并且有报道称AD与5q31存在连锁关系。最近，已报道了IL12B的单核苷酸多态性（SNP）（1188A/C）。在功能方面，有报道称该SNP与IL12B mRNA表达水平相关。为了解该SNP是否与AD或PsV的易感性相关，我们调查了日本人群中AD患者、PsV患者及对照人群中该SNP的基因型和等位基因频率，共检测了164例AD患者、143例PsV患者和100名健康个体。采用聚合酶链反应-限制性片段长度多态性方法进行基因分型。与对照组（50.5%）相比，AD患者中A等位基因频率降低（40.9%，p = 0.031），而PsV患者中A等位基因频率升高（60.1%，p = 0.035）。这表明IL12B SNP可能通过影响Th1/Th2平衡与AD和PsV的易感性相关。
