Imanishi J
Jpn J Microbiol. 1975 Oct;19(5):337-42. doi: 10.1111/j.1348-0421.1975.tb00889.x.
It was found that a preparation of mouse L cell interferon induced by Newcastle disease virus (NDV) possessed not only interferon activity but also inhibitory activity upon migration of guinea pig peritoneal macrophages (MIF activity). These activities were also observed in a preparation of human leukocyte interferon induced by NDV. The interferon and MIF activities shared common characteristics in the dose response, time course of in vitro production, thermal stability, sensitivity to trypsin and periodate, and elution pattern in CM-Sephadex column chromatography. However, gel filtration pattern with Sephadex G-100 showed two separate peaks. Fractions collected from the first peak, corresponding to a molecular weight of about 45 000, had only the MIF activity, while those collected from the second peak, corresponding to a molecular weight of about 30 000, had both the interferon and MIF activities. A preparation of mouse brain interferon induced by Japanese encephalitis virus had a much weaker MIF activity than the L cell interferon, although these preparations were equal in interferon activity (5000 units/ml).
研究发现，新城疫病毒（NDV）诱导产生的小鼠L细胞干扰素制剂不仅具有干扰素活性，而且对豚鼠腹腔巨噬细胞的迁移具有抑制活性（MIF活性）。在NDV诱导产生的人白细胞干扰素制剂中也观察到了这些活性。干扰素和MIF活性在剂量反应、体外产生的时间进程、热稳定性、对胰蛋白酶和高碘酸盐的敏感性以及CM-葡聚糖凝胶柱色谱中的洗脱模式方面具有共同特征。然而，用葡聚糖凝胶G-100进行的凝胶过滤图谱显示有两个分开的峰。从第一个峰收集的级分，对应分子量约为45000，仅具有MIF活性，而从第二个峰收集的级分，对应分子量约为30000，同时具有干扰素和MIF活性。日本脑炎病毒诱导产生的小鼠脑干扰素制剂的MIF活性比L细胞干扰素弱得多，尽管这些制剂的干扰素活性相同（5000单位/毫升）。
