Stewart W E, LeGoff S
J Gen Virol. 1977 Nov;37(2):277-84. doi: 10.1099/0022-1317-37-2-277.
The molecular heterogeneity of acid-stable (Type I) mouse interferons induced in C243 cells by Newcastle disease virus was analysed by SDS-polyacrylamide gel electrophoresis under non-reducing and reducing conditions, and the profiles of antiviral activities obtained were characterized biologicaly in mouse cells and in heterologous (guinea-pig) cells. Two bands of activity, A and B, were consistently present in all interferon preparations tested: under reducing conditions, the activity in all fractions of band A (with a peak of activity at about 38000 daltons) was uniformly increased, while that of band B (with a peak at about 22000 daltons) was uniformly diminished. All the active fraction in band A had only slight activity (less than 10% of homologous titres) on guinea-pig cells, whereas all those in band B were significantly more active an guinea-pig cells than on homologous L cells. Thus, mouse type I interferon preparations contain two molecular populations of interferons that can be distinguished physically (by size), biochemically (by the effect of reduction on reactivation from SDS) and biologically (by activity in heterologous cells).
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，在非还原和还原条件下，分析了新城疫病毒在C243细胞中诱导产生的酸稳定型（I型）小鼠干扰素的分子异质性，并对所获得的抗病毒活性谱在小鼠细胞和异源（豚鼠）细胞中进行了生物学特性鉴定。在所有测试的干扰素制剂中，始终存在两条活性带，A和B：在还原条件下，A带所有组分的活性（活性峰值约为38000道尔顿）均一致增加，而B带（峰值约为22000道尔顿）的活性则一致降低。A带中的所有活性组分对豚鼠细胞只有轻微活性（低于同源滴度的10%），而B带中的所有活性组分在豚鼠细胞上的活性明显高于同源L细胞。因此，小鼠I型干扰素制剂包含两种分子群体的干扰素，它们可以通过物理方法（按大小）、生化方法（通过还原对SDS再活化的影响）和生物学方法（通过在异源细胞中的活性）加以区分。
