Akita Joe, Takahashi Masayo, Hojo Masato, Nishida Akihiro, Haruta Masatoshi, Honda Yoshihito
Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Brain Res. 2002 Nov 8;954(2):286-93. doi: 10.1016/s0006-8993(02)03356-5.
The purpose of this study was to evaluate the effects of the retinal environment and retinoic acid (RA) pretreatment on the differentiation of transplanted adult rat hippocampus-derived neural stem cells (AHSCs). AHSCs were transplanted into embryonic (E18) or neonatal (P6) rat retinal explants and the mixture was cultured for 2 weeks. Other AHSCs were stimulated by 0.5 microM all-trans RA for 6 days before transplantation. Immunofluorescent double staining showed that a larger number of AHSCs became beta-tubulin III-positive neurons in the E18 than in P6 retinas. In addition, many AHSCs became MAP2ab-positive and MAP5-positive neurons following RA pretreatment and transplantation. Only a few AHSCs became HPC-1-, calbindin-, PKC- or rhodopsin-positive cells under these conditions. We conclude that the microenvironment supplied by embryonic retinas is conductive to neuronal differentiation in general. RA stimulation before transplantation was also effective in stimulating differentiation.
本研究的目的是评估视网膜环境和视黄酸（RA）预处理对移植的成年大鼠海马来源神经干细胞（AHSCs）分化的影响。将AHSCs移植到胚胎（E18）或新生（P6）大鼠视网膜外植体中，并将混合物培养2周。其他AHSCs在移植前用0.5 microM全反式视黄酸刺激6天。免疫荧光双重染色显示，与P6视网膜相比，E18视网膜中有更多的AHSCs变成β-微管蛋白III阳性神经元。此外，许多AHSCs在RA预处理和移植后变成MAP2ab阳性和MAP5阳性神经元。在这些条件下，只有少数AHSCs变成HPC-1、钙结合蛋白、蛋白激酶C或视紫红质阳性细胞。我们得出结论，胚胎视网膜提供的微环境总体上有利于神经元分化。移植前的RA刺激在促进分化方面也有效。
