染色质可增强Rad54与Rad51之间的链配对。
Strand pairing by Rad54 and Rad51 is enhanced by chromatin.
作者信息
Alexiadis Vassilios, Kadonaga James T
机构信息
Section of Molecular Biology, University of California, San Diego, La Jolla, California 92093-0347, USA.
出版信息
Genes Dev. 2002 Nov 1;16(21):2767-71. doi: 10.1101/gad.1032102.
Abstract
We investigated the role of chromatin in the catalysis of homologous strand pairing by Rad54 and Rad51. Rad54 is related to the ATPase subunits of chromatin-remodeling factors, whereas Rad51 is related to bacterial RecA. In the absence of superhelical tension, we found that the efficiency of strand pairing with chromatin is >100-fold higher than that with naked DNA. In addition, we observed that Rad54 and Rad51 function cooperatively in the ATP-dependent remodeling of chromatin. These findings indicate that Rad54 and Rad51 have evolved to function with chromatin, the natural substrate, rather than with naked DNA.
摘要
我们研究了染色质在Rad54和Rad51催化同源链配对中的作用。Rad54与染色质重塑因子的ATP酶亚基相关，而Rad51与细菌RecA相关。在没有超螺旋张力的情况下，我们发现与染色质进行链配对的效率比与裸露DNA进行链配对的效率高100倍以上。此外，我们观察到Rad54和Rad51在ATP依赖的染色质重塑中协同发挥作用。这些发现表明，Rad54和Rad51已经进化到与天然底物染色质而非裸露DNA一起发挥作用。
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