通用转录机制对激活剂的募集：Oct-1 POU 结构域/人 U1 八聚体/SNAP190 肽三元复合物的 X 射线结构分析
Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex.
作者信息
Hovde Stacy, Hinkley Craig S, Strong Katie, Brooks Aimee, Gu Liping, Henry R William, Geiger James
机构信息
Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, USA.
出版信息
Genes Dev. 2002 Nov 1;16(21):2772-7. doi: 10.1101/gad.1021002.
Abstract
Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of the Oct-1 POU domain/U1 octamer/SNAP190 peptide complex revealed that SNAP190 makes extensive protein contacts with the Oct-1 POU-specific domain and with the DNA phosphate backbone within the enhancer. Although SNAP190 and OCA-B both interact with the Oct-1 POU domain through the same Oct-1 interface, a single nucleotide within the U1 octamer ablates OCA-B recruitment without compromising activator recruitment by SNAP190.
摘要
人类U1小核RNA基因的转录激活依赖于上游增强子中包含的一个非典型八聚体元件。U1八聚体只能微弱地招募Oct-1 POU结构域，不过，包含SNAP190的Oct-1结合结构域的肽能刺激这种招募。对Oct-1 POU结构域/U1八聚体/SNAP190肽复合物的结构分析表明，SNAP190与Oct-1 POU特异性结构域以及增强子内的DNA磷酸骨架进行广泛的蛋白质接触。尽管SNAP190和OCA-B都通过相同的Oct-1界面与Oct-1 POU结构域相互作用，但U1八聚体内的一个单核苷酸会消除OCA-B的招募，而不会影响SNAP190对激活剂的招募。
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