Jayan Geetha C, Casey John L
Division of Molecular Virology and Immunology, Georgetown University Medical Center, Rockville, Maryland 20850, USA.
J Virol. 2002 Dec;76(23):12399-404. doi: 10.1128/jvi.76.23.12399-12404.2002.
Hepatitis delta virus (HDV) requires host RNA editing at the viral RNA amber/W site. Of the two host genes responsible for RNA editing via deamination of adenosines in double-stranded RNAs, short inhibitory RNA-mediated knockdown of host ADAR1 expression but not that of ADAR2 led to decreased HDV amber/W editing and virus production. Despite substantial sequence and structural variation among the amber/W sites of the three HDV genotypes, ADAR1a was primarily responsible for editing all three. We conclude that ADAR1 is primarily responsible for editing HDV RNA at the amber/W site during HDV infection.
丁型肝炎病毒（HDV）需要宿主对病毒RNA的琥珀色/W位点进行RNA编辑。在负责通过双链RNA中腺苷脱氨作用进行RNA编辑的两个宿主基因中，利用短发夹RNA介导的敲低宿主ADAR1而非ADAR2的表达，导致HDV琥珀色/W编辑和病毒产生减少。尽管三种HDV基因型的琥珀色/W位点存在大量序列和结构变异，但ADAR1a主要负责对所有三种进行编辑。我们得出结论，在HDV感染期间，ADAR1主要负责对HDV RNA的琥珀色/W位点进行编辑。
