Ivaniushina Valeria, Radjef Nadjia, Alexeeva Marfa, Gault Elyanne, Semenov Sergei, Salhi Mohammed, Kiselev Oleg, Dény Paul
Laboratoire de Bactériologie, Virologie - Hygiène, Equipe d'Accueil 'Agents Transmissibles et Hôtes, Signalisation Cellulaire, Oncogenèse', Hôpital Avicenne, UFR Santé Médecine Biologie Humaine, Université Paris 13, 125 route de Stalingrad, 93009, Bobigny cedex, France3.
Influenza Institute, St Petersburg, Russia1.
J Gen Virol. 2001 Nov;82(Pt 11):2709-2718. doi: 10.1099/0022-1317-82-11-2709.
Currently, three genotypes of hepatitis delta virus (HDV) are described. The most common, genotype I, has a worldwide distribution; in contrast, genotype II has been found previously only in Japan and Taiwan, while genotype III is found exclusively in South America. Considering the high prevalence of HDV in Northern Siberia (Russia), restriction fragment length polymorphism (RFLP) was used to analyse HDV genotypes from 29 infected patients living in Yakutia. Of these isolates, 11 were characterized by partial nucleotide sequencing and two isolates were completely sequenced. Phylogenetic inference methods included maximum parsimony, maximum likelihood and distance analyses. A restriction pattern consistent with HDV genotype I was found in 14 samples, while the remaining 15 showed a different restriction pattern, inconsistent with any known genotype. Five Yakutian HDV isolates with the type I restriction pattern were sequenced and confirmed to be affiliated with genotype I, although the phylogenetic results indicate that they were heterogeneous and did not cluster together. Sequencing of eight isolates with the new RFLP pattern revealed that these isolates were most closely related to HDV genotype II. In contrast to HDV Yakutian genotype I sequences, all of these type II sequences formed a well-defined clade on phylogenetic trees. Comparison of clinical presentations during hospitalization between patients infected with HDV type I (n=14) and type II (n=15) did not reveal any differences in the severity of infection. These data indicate that the distribution of genotype II is not restricted to Taiwan or Japan, but spreads over Northern Asia, appearing in the native population of Yakutia. Type II Yakutian strains appeared to form a well-defined subclade and could be associated with severe chronic hepatitis in this area.
目前，已描述了三种丁型肝炎病毒（HDV）基因型。最常见的基因型I在全球范围内均有分布；相比之下，基因型II此前仅在日本和台湾被发现，而基因型III仅在南美洲被发现。考虑到HDV在俄罗斯北西伯利亚地区的高流行率，采用限制性片段长度多态性（RFLP）分析了29名生活在雅库特的感染患者的HDV基因型。在这些分离株中，11株通过部分核苷酸测序进行了特征分析，2株进行了全序列测定。系统发育推断方法包括最大简约法、最大似然法和距离分析。在14个样本中发现了与HDV基因型I一致的限制性图谱，而其余15个样本显示出不同的限制性图谱，与任何已知基因型均不一致。对5株具有I型限制性图谱的雅库特HDV分离株进行测序，证实它们属于基因型I，尽管系统发育结果表明它们是异质的，并未聚类在一起。对8株具有新RFLP图谱的分离株进行测序，结果显示这些分离株与HDV基因型II关系最为密切。与雅库特HDV基因型I序列不同，所有这些II型序列在系统发育树上形成了一个明确的分支。对感染I型HDV（n = 14）和II型HDV（n = 15）的患者住院期间的临床表现进行比较，未发现感染严重程度有任何差异。这些数据表明，基因型II的分布并不局限于台湾或日本，而是分布在北亚地区，出现在雅库特的当地人群中。雅库特II型毒株似乎形成了一个明确的亚分支，可能与该地区的严重慢性肝炎有关。
