Potanova O L, Smirnova I P, Turchinskiĭ V V, Berezov T T
Vopr Med Khim. 1992 Sep-Oct;38(5):28-30.
A spectrophotometric procedure for estimation of L-lysine-alpha-oxidase activity involved measurement of optical density in samples after reaction of the H2O2 formed with o-phenylene diamine. Suitable values of pH, o-phenylene diamine concentration and time of incubation were chosen. The procedure developed was distinctly dissimilar as compared with the methods currently widely used. Advantages of the new method are due to its sensitivity, reproducibility, accessibility, relative safety and short time of the reaction performance. The procedure may be used for estimation of L-lysine content in various biological materials.
一种用于测定L-赖氨酸-α-氧化酶活性的分光光度法，该方法涉及在用邻苯二胺与生成的过氧化氢反应后测量样品中的光密度。选择了合适的pH值、邻苯二胺浓度和孵育时间。与目前广泛使用的方法相比，所开发的方法明显不同。新方法的优点在于其灵敏度、可重复性、易操作性、相对安全性以及反应性能所需的时间短。该方法可用于测定各种生物材料中的L-赖氨酸含量。
