Polat M Fevzi, Taysi Seyithan, Gul Mustafa, Cikman Oztekin, Yilmaz Ismail, Bakan Ebubekir, Erdogan Fazli
Biotechnology Application and Research Center, Ataturk University, 25240 Erzurum, Turkey.
Cell Biochem Funct. 2002 Dec;20(4):327-31. doi: 10.1002/cbf.980.
The aim of this study was to investigate the alterations in lipid peroxidation and antioxidant enzyme defences in the blood of patients with malignant breast tumour and benign breast disease. Forty patients with malignant breast tumour, 20 patients with benign breast disease and also 20 healthy control subjects were recruited for the study. Malondialdehyde levels in plasma and erythrocytes, and the activities of erythrocyte CuZn-superoxide dismutase, catalase, glutathione peroxidase and glucose-6-phosphate dehydrogenase were measured. Malondialdehyde levels were higher in patients with both benign breast disease and malignant breast tumour compared with control subjects. The activities of all antioxidant enzymes were higher in patients with malignant breast tumour, while only glutathione peroxidase and CuZn-superoxide dismutase activities were higher in patients with benign breast disease. Except for glucose-6-phosphate dehydrogenase, the antioxidant enzymes studied correlated positively with the malondialdehyde levels in patients with malignant breast tumour. On the other hand, only glucose-6-phosphate dehydrogenase activity was increased by the level of malignancy. The activity increases in erythrocyte antioxidant enzymes may be a compensatory upregulation in response to increased oxidative stress especially in patients with malignant breast tumour.
本研究旨在调查恶性乳腺肿瘤患者和良性乳腺疾病患者血液中脂质过氧化和抗氧化酶防御系统的变化。本研究招募了40例恶性乳腺肿瘤患者、20例良性乳腺疾病患者以及20名健康对照者。检测了血浆和红细胞中的丙二醛水平，以及红细胞铜锌超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和葡萄糖-6-磷酸脱氢酶的活性。与对照者相比，良性乳腺疾病患者和恶性乳腺肿瘤患者的丙二醛水平均较高。恶性乳腺肿瘤患者所有抗氧化酶的活性均较高，而良性乳腺疾病患者只有谷胱甘肽过氧化物酶和铜锌超氧化物歧化酶的活性较高。在恶性乳腺肿瘤患者中，除葡萄糖-6-磷酸脱氢酶外，所研究的抗氧化酶与丙二醛水平呈正相关。另一方面，只有葡萄糖-6-磷酸脱氢酶的活性随恶性程度的增加而升高。红细胞抗氧化酶活性的增加可能是对氧化应激增加的一种代偿性上调，尤其是在恶性乳腺肿瘤患者中。
