Sbizera Maria Carolina Ricciardi, Barreto José Victor Pronievicz, Pertile Simone Fernanda Nedel, de Almeida Rego Fabíola Cristine, Lisbôa Julio Augusto Naylor, da Cunha Filho Luiz Fernando Coelho
Post Graduate Program in Animal Health and Production, Department of Agrarian Sciences, Universidade Pitágoras Unopar Anhanguera, Arapongas, Brazil.
Post Graduate Program in Animal Science, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Brazil.
Braz J Microbiol. 2024 Dec;55(4):4191-4198. doi: 10.1007/s42770-024-01486-9. Epub 2024 Aug 23.
The Bluetongue disease is an infectious and non-contagious viral disease mainly transmitted through hematophagous vector of the Culicoides genus, to domestic and wild ruminants. The aim of this study was to evaluate the antibodies occurrence, persistence and potential risk factors associated with bluetongue virus infection in sheep flocks in the state of Parana, Brazil. The competitive ELISA test was applied to evaluate 690 blood serum samples from 22 farms in eight mesoregions of Parana in 2014, and 270 sheep blood serum samples from 10 of the 22 previously studied farms in 2017. A questionnaire was applied to evaluate the risk factors associated with BTV infection. In 2014 and 2017, the numbers of seroreactive sheep were found to be 28.26% (195/690) and 41.11% (111/270), respectively, representing 95.45% (21/22) and 90% (9/10) of the flocks. The significant variables considered as risk factors were Culicoides presence (P < 0.0001; OR = 8.83 and 95% CI 4.28-18.22); genealogical record (P < 0.0001; OR = 0.23 and 95% CI 0.12-0.45) and use of sheepfold (P = 0.0208; OR = 0.36 and 95% CI 0.15-0 0.86). It was determined that BTV infection is endemic in Parana and persists in the mesoregions where the climate is favorable to vector proliferation.
蓝舌病是一种传染性但非接触性的病毒性疾病，主要通过库蠓属吸血媒介传播给家养和野生反刍动物。本研究的目的是评估巴西巴拉那州羊群中与蓝舌病病毒感染相关的抗体出现情况、持续性及潜在风险因素。2014年，采用竞争ELISA试验对巴拉那州八个中区域22个农场的690份血清样本进行评估，2017年对之前研究的22个农场中10个农场的270份绵羊血清样本进行评估。应用问卷调查评估与蓝舌病病毒感染相关的风险因素。2014年和2017年，血清反应阳性绵羊的数量分别为28.26%（195/690）和41.11%（111/270），分别占羊群的95.45%（21/22）和90%（9/10）。被视为风险因素的显著变量有库蠓的存在（P＜0.0001；OR = 8.83，95%可信区间4.28 - 18.22）；系谱记录（P＜0.0001；OR = 0.23，95%可信区间0.12 - 0.45）和羊舍的使用情况（P = 0.0208；OR = 0.36，95%可信区间0.15 - 0.86）。确定蓝舌病病毒感染在巴拉那州为地方病，并在气候有利于媒介繁殖的中区域持续存在。
