Kim E V, Chuvakova Z K, Isaeva E S, Mikhaĭlov G G, Biriukova T B
Vopr Virusol. 1975 Nov-Dec(6):732-4.
An effective method for isolation of influenza virus from the nasopharyngeal specimens of patients is described consisting of interruption of the virus reproduction cycle in chorioallantoic membranes of chick embryos (CECAM) at early stages of development of the infection followed by inoculation of infected CECAM homogenate into a fresh homologous medium. The new method of virus isolation is superior to the conventional one by a higher isolation rate, economy of material and time saving which is important for identification of influenza outbreaks.
本文描述了一种从患者鼻咽标本中分离流感病毒的有效方法，该方法包括在感染发展的早期阶段阻断鸡胚绒毛尿囊膜（CECAM）中的病毒繁殖周期，然后将感染的CECAM匀浆接种到新鲜的同源培养基中。这种新的病毒分离方法在分离率、材料经济性和节省时间方面优于传统方法，这对于流感疫情的识别很重要。
