Proliferation of mouse ascites mammary carcinoma MM2 cells in the primary culture.

Culture conditions suitable for active proliferation in primary culture of transplantable mouse ascites mammary carcinoma MM2 cells were investigated; kind of serum, its optimal concentration, composition of medium, inoculum size of cells, addition of ascites, conditioned medium or hormones, and others. The most active growth was obtained in the medium consisting of 5% fetal calf serum and 95% RPMI-1640, so far as examined. The addition of 1 mug/ml of estriol more accelerated the proliferation. MM2 cells were found to have preserved their tumorigenicity after 5 months of cultivation.