Ng Jocelyn H, Ilag Leodevico L
Xerion Pharmaceuticals AG, Martinsried, Germany.
J Cell Mol Med. 2002 Jul-Sep;6(3):329-40. doi: 10.1111/j.1582-4934.2002.tb00512.x.
The development of microchips involving proteins has accelerated within the past few years. Although DNA chip technologies formed the precedent, many different strategies and technologies have been used because proteins are inherently a more complex type of molecule. This review covers the various biomedical applications of protein chips in diagnostics, drug screening and testing, disease monitoring, drug discovery (proteomics), and medical research. The proteomics and drug discovery section is further subdivided to cover drug discovery tools (on-chip separations, expression profiling, and antibody arrays), molecular interactions and signaling pathways, the identification of protein function, and the identification of novel therapeutic compounds. Although largely focused on protein chips, this review includes chips involving cells and tissues as a logical extension of the type of data that can be generated from these microchips.
在过去几年中，涉及蛋白质的微芯片发展加速。尽管DNA芯片技术开创了先例，但由于蛋白质本质上是一种更为复杂的分子类型，人们已采用了许多不同的策略和技术。本综述涵盖了蛋白质芯片在诊断、药物筛选与测试、疾病监测、药物发现（蛋白质组学）及医学研究等方面的各种生物医学应用。蛋白质组学与药物发现部分进一步细分，涵盖药物发现工具（芯片上的分离、表达谱分析及抗体阵列）、分子相互作用和信号通路、蛋白质功能鉴定以及新型治疗化合物的鉴定。尽管本综述主要聚焦于蛋白质芯片，但也包括了涉及细胞和组织的芯片，作为这些微芯片所能产生的数据类型的合理延伸。
