Glasheen B M, Polashock J J, Lawrence D M, Gillett J M, Ramsdell D C, Vorsa N, Hillman B I
Department of Plant Biology and Pathology, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA.
Arch Virol. 2002 Nov;147(11):2169-86. doi: 10.1007/s00705-002-0866-7.
The double-stranded DNA genome of Blueberry red ringspot virus (BRRV), a member of the family Caulimoviridae, was cloned and sequenced. The genome organization and relationships of the 8303 nt sequence revealed BRRV to be a tentative member of the genus that has been provisionally named "Soybean chlorotic mottle-like viruses", rather than a member of the genus Caulimovirus, in which it had been placed previously. Insertion of the putative 35S promoter homolog of BRRV into promoterless constructs carrying the UidA (beta-glucuronidase) gene resulted in high-level transient expression from cranberry and stable expression from transgenic tobacco. Sequences of 5'-RACE clones derived from transcripts from transgenic tobacco were consistent with the map position of the promoter.
花椰菜花叶病毒科成员蓝莓红环斑病毒（BRRV）的双链DNA基因组被克隆并测序。8303个核苷酸序列的基因组结构和关系表明，BRRV是暂定名为“大豆褪绿斑驳样病毒”属的一个暂定成员，而不是先前归类的花椰菜花叶病毒属成员。将BRRV假定的35S启动子同源物插入携带UidA（β-葡萄糖醛酸酶）基因的无启动子构建体中，导致越橘中高水平的瞬时表达和转基因烟草中的稳定表达。来自转基因烟草转录本的5'-RACE克隆序列与启动子的图谱位置一致。
