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波兰蓝莓红环斑病毒分离株的分子特征

Molecular characterization of Polish Blueberry red ringspot virus isolate.

作者信息

Kalinowska E, Paduch-Cichal E, Chodorska M

机构信息

Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland.

出版信息

Virus Genes. 2012 Apr;44(2):309-11. doi: 10.1007/s11262-011-0679-4. Epub 2011 Oct 11.

DOI:10.1007/s11262-011-0679-4
PMID:21987074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3289790/
Abstract

In this study, we determined the complete sequence of the genomic DNA of a Polish isolate of Blueberry red ringspot virus (BRRSV24) and compared it with a Czech (Darrow 5), and the US isolates of the virus and those of other Caulimoviridae family. The genomic DNA of BRRSV24 consists of 8,265 nucleotides and encodes eight open reading frames (ORFs). The sequence homologies of the eight ORFs of BRRSV24 were from 95 to 98% in respect of Darrow 5 and from 91 to 98% in respect of the US isolates at the amino acid level. This high level of amino acid sequence identity within the coding regions among the Czech, the US and Polish BRRSV isolates is suggestive of their common origin.

摘要

在本研究中,我们测定了波兰蓝莓红环斑病毒分离株(BRRSV24)基因组DNA的完整序列,并将其与捷克分离株(Darrow 5)、美国分离株以及花椰菜花叶病毒科其他病毒的分离株进行了比较。BRRSV2基因组DNA由8265个核苷酸组成,编码8个开放阅读框(ORF)。BRRSV24的8个ORF在氨基酸水平上与Darrow 5的序列同源性为95%至98%,与美国分离株的序列同源性为91%至98%。捷克、美国和波兰的BRRSV分离株编码区域内如此高的氨基酸序列同一性表明它们有共同的起源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3723/3289790/dc4cc6bdaf43/11262_2011_679_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3723/3289790/dc4cc6bdaf43/11262_2011_679_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3723/3289790/dc4cc6bdaf43/11262_2011_679_Fig1_HTML.jpg

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本文引用的文献

1
Molecular Detection and Discrimination of Blueberry red ringspot virus Strains Causing Disease in Cultivated Blueberry and Cranberry.对栽培蓝莓和蔓越莓致病的蓝莓红环斑病毒株系的分子检测与鉴别
Plant Dis. 2009 Jul;93(7):727-733. doi: 10.1094/PDIS-93-7-0727.
2
First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic.捷克共和国高丛蓝莓中蓝莓红环斑病毒的首次报道
Plant Dis. 2010 Aug;94(8):1071. doi: 10.1094/PDIS-94-8-1071B.
3
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
MEGA5:用于最大似然法、进化距离法和最大简约法的分子进化遗传学分析。
Mol Biol Evol. 2011 Oct;28(10):2731-9. doi: 10.1093/molbev/msr121. Epub 2011 May 4.
4
Cloning, sequencing, and promoter identification of Blueberry red ringspot virus, a member of the family Caulimoviridae with similarities to the "Soybean chlorotic mottle-like" genus.蓝莓红环斑病毒的克隆、测序及启动子鉴定,该病毒属于花椰菜花叶病毒科,与“大豆褪绿斑驳样”属具有相似性。
Arch Virol. 2002 Nov;147(11):2169-86. doi: 10.1007/s00705-002-0866-7.